論文 - 詳細
| RRC ID | 67 |
|---|---|
| 著者 | Takeuchi K, Sereemaspun A, Inagaki T, Hakamata Y, Kaneko T, Murakami T, Takahashi M, Kobayashi E, Ookawara S. |
| タイトル | Morphologic characterization of green fluorescent protein in embryonic, neonatal, and adult transgenic rats. |
| ジャーナル | Anat Rec A Discov Mol Cell Evol Biol |
| Abstract |
Transgenic (Tg) animals with reporter genes are useful models in which to study cell lineage and the process of differentiation into tissues. We developed the green fluorescent protein (GFP)-Tg rat, which is more suitable for transplantation and stem cell research because it is larger than mice. We found that marker gene expression was dependent on each organ and developmental stage. In this study we describe GFP expression in various tissues from embryonic, neonatal, and adult animals. GFP expression in brain, lung, liver, and islet tissues was restricted to early developmental stages, but it was continuously strong in the exocrine pancreas, kidney, and cardiac and skeletal muscles. The CAG promoter that was presumed to induce ubiquitous protein expression might be responsible for the differences in expression. |
| 巻・号 | 274(2) |
| ページ | 883-6 |
| 公開日 | 2003-10-1 |
| DOI | 10.1002/ar.a.10111 |
| PMID | 12973712 |
| MeSH | Animals Animals, Genetically Modified* / metabolism Animals, Newborn / metabolism* Embryo, Mammalian / metabolism* Gene Expression Genes, Reporter Genetic Markers Green Fluorescent Proteins Indicators and Reagents* Luminescent Proteins / genetics Luminescent Proteins / metabolism* Organogenesis Promoter Regions, Genetic Rats Rats, Wistar Tissue Distribution |
| 引用数 | 21 |
| WOS 分野 | ANATOMY & MORPHOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ラット | W-Tg(CAG-GFP)184Ys(strainID=525) |