RRC ID 67058
Author Masutani T, Yamada S, Hara A, Takahashi T, Green PG, Niwa M.
Title Exogenous Application of Proteoglycan to the Cell Surface Microenvironment Facilitates to Chondrogenic Differentiation and Maintenance.
Journal Int J Mol Sci
Abstract Osteoarthritis (OA), a disease that greatly impacts quality of life, has increasing worldwide prevalence as the population ages. However, its pathogenic mechanisms have not been fully elucidated and current therapeutic treatment strategies are inadequate. In recent years, abnormal endochondral ossification in articular cartilage has received attention as a pathophysiological mechanism in OA. Cartilage is composed of abundant extracellular matrix components, which are involved in tissue maintenance and regeneration, but how these factors affect endochondral ossification is not clear. Here, we show that the application of aggrecan-type proteoglycan from salmon nasal cartilage (sPG) exhibited marked proliferative capacity through receptor tyrosine kinases in chondroprogenitor cells, and also exhibited differentiation and three-dimensional structure formation via phosphorylation of Insulin-like Growth Factor-1 Receptor and Growth Differentiation Factor 5 expression. Furthermore, sPG inhibited calcification via expression of Runx2 and Col10 (factors related to induction of calcification), while increasing Mgp, a mineralization inhibitory factor. As a result of analyzing the localization of sPG applied to the cells, it was localized on the surface of the cell membrane. In this study, we found that sPG, as a biomaterial, could regulate cell proliferation, differentiation and calcification inhibition by acting on the cell surface microenvironment. Therefore, sPG may be the foundation for a novel therapeutic approach for cartilage maintenance and for improved symptoms in OA.
Volume 21(20)
Published 2020-10-19
DOI 10.3390/ijms21207744
PII ijms21207744
PMID 33086766
PMC PMC7589071
MeSH Calcification, Physiologic / drug effects Cartilage, Articular / metabolism Cell Differentiation* / drug effects Cell Membrane / drug effects Cell Membrane / metabolism* Cell Proliferation / drug effects Cell Survival / drug effects Cells, Cultured Cellular Microenvironment* / drug effects Chondrogenesis* / drug effects ErbB Receptors / metabolism Extracellular Matrix / drug effects Extracellular Matrix / metabolism Humans Proteoglycans / pharmacology* RNA, Messenger / genetics RNA, Messenger / metabolism Receptor, IGF Type 1 / metabolism
IF 4.556
Human and Animal Cells ATDC5(RCB0565)