論文 - 詳細
| RRC ID | 67182 |
|---|---|
| 著者 | Melo SA, Luecke LB, Kahlert C, Fernandez AF, Gammon ST, Kaye J, LeBleu VS, Mittendorf EA, Weitz J, Rahbari N, Reissfelder C, Pilarsky C, Fraga MF, Piwnica-Worms D, Kalluri R. |
| タイトル | Glypican-1 identifies cancer exosomes and detects early pancreatic cancer. |
| ジャーナル | Nature |
| Abstract |
Exosomes are lipid-bilayer-enclosed extracellular vesicles that contain proteins and nucleic acids. They are secreted by all cells and circulate in the blood. Specific detection and isolation of cancer-cell-derived exosomes in the circulation is currently lacking. Using mass spectrometry analyses, we identify a cell surface proteoglycan, glypican-1 (GPC1), specifically enriched on cancer-cell-derived exosomes. GPC1(+) circulating exosomes (crExos) were monitored and isolated using flow cytometry from the serum of patients and mice with cancer. GPC1(+) crExos were detected in the serum of patients with pancreatic cancer with absolute specificity and sensitivity, distinguishing healthy subjects and patients with a benign pancreatic disease from patients with early- and late-stage pancreatic cancer. Levels of GPC1(+) crExos correlate with tumour burden and the survival of pre- and post-surgical patients. GPC1(+) crExos from patients and from mice with spontaneous pancreatic tumours carry specific KRAS mutations, and reliably detect pancreatic intraepithelial lesions in mice despite negative signals by magnetic resonance imaging. GPC1(+) crExos may serve as a potential non-invasive diagnostic and screening tool to detect early stages of pancreatic cancer to facilitate possible curative surgical therapy. |
| 巻・号 | 523(7559) |
| ページ | 177-82 |
| 公開日 | 2015-7-9 |
| DOI | 10.1038/nature14581 |
| PII | nature14581 |
| PMID | 26106858 |
| PMC | PMC4825698 |
| MeSH | Animals Biomarkers / blood Cell Line, Tumor Exosomes / genetics Exosomes / metabolism* Female Glypicans* / blood Glypicans* / metabolism HCT116 Cells Humans MCF-7 Cells Male Mice NIH 3T3 Cells Pancreatic Neoplasms / blood Pancreatic Neoplasms / diagnosis* Pancreatic Neoplasms / pathology Proto-Oncogene Proteins / metabolism Proto-Oncogene Proteins p21(ras) ras Proteins / metabolism |
| IF | 42.779 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Peer-review(Publons) |
| 各媒体での言及数の合計 | 580 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | T3M-4(RCB1021) |