Reference - Detail
| RRC ID | 67196 |
|---|---|
| Author | Wu D, Yan J, Shen X, Sun Y, Thulin M, Cai Y, Wik L, Shen Q, Oelrich J, Qian X, Dubois KL, Ronquist KG, Nilsson M, Landegren U, Kamali-Moghaddam M. |
| Title | Profiling surface proteins on individual exosomes using a proximity barcoding assay. |
| Journal | Nat Commun |
| Abstract |
Exosomes have been implicated in numerous biological processes, and they may serve as important disease markers. Surface proteins on exosomes carry information about their tissues of origin. Because of the heterogeneity of exosomes it is desirable to investigate them individually, but this has so far remained impractical. Here, we demonstrate a proximity-dependent barcoding assay to profile surface proteins of individual exosomes using antibody-DNA conjugates and next-generation sequencing. We first validate the method using artificial streptavidin-oligonucleotide complexes, followed by analysis of the variable composition of surface proteins on individual exosomes, derived from human body fluids or cell culture media. Exosomes from different sources are characterized by the presence of specific combinations of surface proteins and their abundance, allowing exosomes to be separately quantified in mixed samples to serve as markers for tissue-specific engagement in disease. |
| Volume | 10(1) |
| Pages | 3854 |
| Published | 2019-8-26 |
| DOI | 10.1038/s41467-019-11486-1 |
| PII | 10.1038/s41467-019-11486-1 |
| PMID | 31451692 |
| PMC | PMC6710248 |
| MeSH | Body Fluids / cytology Cell Line, Tumor DNA, Single-Stranded / genetics DNA, Single-Stranded / metabolism Exosomes / metabolism* Gene Expression Profiling / methods* High-Throughput Nucleotide Sequencing / methods Humans Immunoconjugates / genetics Immunoconjugates / metabolism Membrane Proteins / genetics Membrane Proteins / metabolism* Molecular Probes / genetics Molecular Probes / metabolism Oligonucleotides / genetics Oligonucleotides / metabolism Sequence Analysis, DNA / methods |
| IF | 12.121 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 65 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | MKN7(RCB0999) |