Reference - Detail
| RRC ID | 6719 |
|---|---|
| Author | Choi YK, Park JS, Kong JS, Morio T, Park YS. |
| Title | D-threo-tetrahydrobiopterin is synthesized via 1'-oxo-2'-D-hydroxypropyl-tetrahydropterin in Dictyostelium discoideum Ax2. |
| Journal | FEBS Lett |
| Abstract |
The biosynthesis of D-threo-tetrahydrobiopterin (DH4, tetrahydrodictyopterin) in Dictyostelium discoideum Ax2 was investigated through the mutant disrupted in the gene encoding sepiapterin reductase (SR) by insertional inactivation. The mutant cells, being completely devoid of SR protein, showed 18.1% of L-erythro-tetrahydrobiopterin (BH4) and 0.6% of DH4 productions in the wild type cells. The mutant cells were also identified to excrete D- and L-sepiapterin, which were presumed to originate from intracellular 1'-oxo-2'-D-hydroxypropyl- and 1'-oxo-2'-L-hydroxypropyl-tetrahydropterin (H4-pterin), respectively. Furthermore, in a coupled assay with Dictyostelium SR, the mutant cell extract exhibited a novel enzyme activity converting 6-pyruvoyltetrahydropterin to 1'-oxo-2'-D-hydroxypropyl-H4-pterin. These results are clear demonstration of the in vivo synthesis of DH4 via 1'-oxo-2'-D-hydroxypropyl-H4-pterin as well as an alternative synthesis of BH4 and DH4 in the complete absence of SR. |
| Volume | 579(14) |
| Pages | 3085-9 |
| Published | 2005-6-6 |
| DOI | 10.1016/j.febslet.2005.04.064 |
| PII | S0014-5793(05)00547-8 |
| PMID | 15896778 |
| MeSH | Alcohol Oxidoreductases / genetics Alcohol Oxidoreductases / metabolism Animals Biopterin / analogs & derivatives* Biopterin / chemistry Biopterin / metabolism* Chromatography, High Pressure Liquid Dictyostelium / chemistry Dictyostelium / classification Dictyostelium / genetics Dictyostelium / metabolism* Molecular Structure Mutation / genetics Pteridines / metabolism Pterins / chemistry Pterins / metabolism* |
| IF | 3.057 |
| Times Cited | 10 |
| WOS Category | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
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| Total number of mentions | 0 |
| Resource | |
| Cellular slime molds | |