RRC ID |
67246
|
Author |
Togami K, Miyao A, Miyakoshi K, Kanehira Y, Tada H, Chono S.
|
Title |
Efficient delivery to human lung fibroblasts (WI-38) of pirfenidone incorporated into liposomes modified with truncated basic fibroblast growth factor and its inhibitory effect on collagen synthesis in idiopathic pulmonary fibrosis.
|
Journal |
Biol Pharm Bull
|
Abstract |
In the present in vitro study, we assessed the delivery of pirfenidone incorporated into liposomes modified with truncated basic fibroblast growth factor (tbFGF) to lung fibroblasts and investigated the anti-fibrotic effect of the drug. The tbFGF peptide, KRTGQYKLC, was used to modify the surface of liposomes (tbFGF-liposomes). We used the thin-layer evaporation method, followed by sonication, to prepare tbFGF-liposomes containing pirfenidone. The cellular accumulation of tbFGF-liposomes was 1.7-fold greater than that of non-modified liposomes in WI-38 cells used as a model of lung fibroblasts. Confocal laser scanning microscopy showed that tbFGF-liposomes were widely localized in WI-38 cells. The inhibitory effects of pirfenidone incorporated into tbFGF-liposomes on transforming growth factor-β1 (TGF-β1)-induced collagen synthesis in WI-38 cells were evaluated by measuring the level of intracellular hydroxyproline, a major component of the protein collagen. Pirfenidone incorporated into tbFGF-liposomes at concentrations of 10, 30, and 100 µM significantly decreased the TGF-β1-induced hydroxyproline content in WI-38 cells. The anti-fibrotic effect of pirfenidone incorporated into tbFGF-liposomes was enhanced compared with that of pirfenidone solution. These results indicate that tbFGF-liposomes are a useful drug delivery system of anti-fibrotic drugs to lung fibroblasts for the treatment of idiopathic pulmonary fibrosis.
|
Volume |
38(2)
|
Pages |
270-6
|
Published |
2015-1-1
|
DOI |
10.1248/bpb.b14-00659
|
PMID |
25747986
|
MeSH |
Cell Line
Cell Proliferation / drug effects
Collagen / metabolism
Fibroblast Growth Factors / administration & dosage*
Fibroblast Growth Factors / chemistry*
Fibroblasts / drug effects*
Fibroblasts / metabolism
HEK293 Cells
Humans
Idiopathic Pulmonary Fibrosis / drug therapy
Idiopathic Pulmonary Fibrosis / metabolism
Liposomes
Lung / cytology
Pyridones / administration & dosage*
|
IF |
1.863
|
Resource |
Human and Animal Cells |
293(RCB1637)
WI-38(RCB0702) |