Reference - Detail
| RRC ID | 6724 |
|---|---|
| Author | Anjard C, Loomis WF. |
| Title | Peptide signaling during terminal differentiation of Dictyostelium. |
| Journal | Proc Natl Acad Sci U S A |
| Abstract |
A wide variety of mechanisms have evolved for intercellular communication in metazoans, but some of the signaling molecules were already used in their predecessors. The social amoeba, Dictyostelium discoideum, is known to use peptides to trigger sporulation within fruiting bodies, but their sequences have not been defined. We found that the peptide signal spore differentiation factor 2 (SDF-2) is processed from acyl-CoA binding protein, AcbA. The mammalian homolog of AcbA is processed to diazepam binding inhibitor that binds to the GABA(A) receptor in the brain and to peripheral 1,4 benzodiazepine receptors. Although Dictyostelium has neither GABA(A) nor peripheral-type benzodiazepine receptors, we find that both a diazepam binding inhibitor peptide and diazepam (Valium) can mimic SDF-2 in a Dictyostelium bioassay. Mutants lacking AcbA sporulate well only when developed in chimeras with WT cells. Using a yeast system we show that ligand binding to the SDF-2 receptor histidine kinase, DhkA, inhibits phosphorelay, which can account for its ability to induce rapid sporulation. |
| Volume | 102(21) |
| Pages | 7607-11 |
| Published | 2005-5-24 |
| DOI | 10.1073/pnas.0501820102 |
| PII | 0501820102 |
| PMID | 15897458 |
| PMC | PMC1140433 |
| MeSH | Amino Acid Sequence Animals Biological Assay Carbon Radioisotopes Cloning, Molecular Diazepam / metabolism Diazepam Binding Inhibitor / metabolism* Dictyostelium / metabolism Dictyostelium / physiology* Genetic Vectors Intercellular Signaling Peptides and Proteins Ligands Molecular Sequence Data Mutation / genetics Peptides / metabolism* Protein Kinases / genetics Protein Kinases / metabolism Reproduction / physiology Signal Transduction / physiology* Yeasts |
| IF | 9.412 |
| Times Cited | 67 |
| WOS Category | CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Cellular slime molds | G01682 |