論文 - 詳細
| RRC ID | 67283 |
|---|---|
| 著者 | Nakai K, Shiga T, Yasuhara R, Sarkar AK, Abe Y, Nakamura S, Hoashi Y, Kotani K, Tatsumoto S, Ishikawa H, Go Y, Inoue T, Mishima K, Akamatsu W, Baba K. |
| タイトル | In vitro monitoring of HTR2A-positive neurons derived from human-induced pluripotent stem cells. |
| ジャーナル | Sci Rep |
| Abstract |
The serotonin 5-HT2A receptor (5-HT2AR) has been receiving increasing attention because its genetic variants have been associated with a variety of neurological diseases. To elucidate the pathogenesis of the neurological diseases associated with 5-HT2AR gene (HTR2A) variants, we have previously established a protocol to induce HTR2A-expressing neurons from human-induced pluripotent stem cells (hiPSCs). Here, we investigated the maturation stages and electrophysiological properties of HTR2A-positive neurons induced from hiPSCs and constructed an HTR2A promoter-specific reporter lentivirus to label the neurons. We found that neuronal maturity increased over time and that HTR2A expression was induced at the late stage of neuronal maturation. Furthermore, we demonstrated successful labelling of the HTR2A-positive neurons, which had fluorescence and generated repetitive action potentials in response to depolarizing currents and an inward current during the application of TCB-2, a selective agonist of 5-HT2ARs, respectively. These results indicated that our in vitro model mimicked the in vivo dynamics of 5-HT2AR. Therefore, in vitro monitoring of the function of HTR2A-positive neurons induced from hiPSCs could help elucidate the pathophysiological mechanisms of neurological diseases associated with genetic variations of the HTR2A gene. |
| 巻・号 | 11(1) |
| ページ | 15437 |
| 公開日 | 2021-7-29 |
| DOI | 10.1038/s41598-021-95041-3 |
| PII | 10.1038/s41598-021-95041-3 |
| PMID | 34326453 |
| PMC | PMC8322101 |
| MeSH | Action Potentials / genetics Adult Blood Donors Cells, Cultured Healthy Volunteers Humans Induced Pluripotent Stem Cells / cytology* Neurogenesis / genetics* Neurons / cytology* Neurons / metabolism* Patch-Clamp Techniques / methods Promoter Regions, Genetic Receptor, Serotonin, 5-HT2A / genetics Receptor, Serotonin, 5-HT2A / metabolism* Signal Transduction / genetics* Transfection |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | SK-N-SH(RCB0426) |