論文 - 詳細
| RRC ID | 67331 |
|---|---|
| 著者 | Oki K, Yoshihara S, Urushihata N, Ghazizadeh M. |
| タイトル | Anti-fibrotic effect of adipose-derived mesenchymal stem cell conditioned medium in muscle fibrosis. |
| ジャーナル | Eur Rev Med Pharmacol Sci |
| Abstract |
OBJECTIVE:Recent investigations have demonstrated that the administration of MSC in mouse model of diseases provided beneficial effects. On the other hand, human adipose-derived MSC condition medium (ADSC-CM) is reported as containing beneficial secreted factors, but its role in muscle fibrosis has not been identified. The aim of this study was to investigate the inhibitory effects of MSC-CM in muscle fibrosis in vitro using the C2C12 murine muscle, myoblast cell line. MATERIALS AND METHODS:C2C12 cells were cultured overnight in 0.1% albumin-Dulbecco's Modifies Eagle's Medium (DMEM). The cells were then pre-incubated in ADSC-CM for 20 min, treated with 2.5-10 ng/mL human TGFβ1 for 8-72 hours and analyzed using RT-qPCR, Western blot and immunofluorescent staining. RESULTS:Treatment with 20% ADSC-CM for 3 days suppressed αSMA protein expression in TGFβ1 treated C2C12 cells. ADSC-CM stimulated the proliferation of C2C12 cells in a dose-dependent manner. Furthermore, TGFβ1 induced Acta2/αSMA mRNA expression which was inhibited by ADSC-CM treatment for 8 hours. Decorin, one of the dermatan sulfate proteoglycans and an endogenous inhibitor of TGFβ1, was expressed in ADSC-CM, but not in TGFβ1 pre-incubated ADSC-CM. CONCLUSIONS:Our studies provide useful information for establishing anti-fibrotic mechanism(s) of ADSC-CM, thus facilitating potential application to prevent muscle fibrosis. |
| 巻・号 | 25(15) |
| ページ | 4953-4963 |
| 公開日 | 2021-8-1 |
| DOI | 10.26355/eurrev_202108_26452 |
| PII | 26452 |
| PMID | 34355367 |
| MeSH | Animals Antifibrotic Agents / pharmacology* Cell Proliferation / drug effects Cells, Cultured Culture Media, Conditioned / pharmacology* Fibrosis / drug therapy* Mesenchymal Stem Cells / drug effects* Mice |
| IF | 3.024 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |