| RRC ID |
67376
|
| 著者 |
Tahara K, Ban T, Minegishi T, Kohn LD.
|
| タイトル |
Immunoglobulins from Graves' disease patients interact with different sites on TSH receptor/LH-CG receptor chimeras than either TSH or immunoglobulins from idiopathic myxedema patients.
|
| ジャーナル |
Biochem Biophys Res Commun
|
| Abstract |
To examine the identity of binding sites for thyrotropin (TSH) and thyroid stimulating antibodies (TSAbs) associated with Graves' disease, we constructed eight human TSH receptor/rat LH-CG receptor chimeras. Substitution of amino acid residues 8-165 of the TSH receptor with the corresponding LH-CG receptor segment (Mc1 + 2) results in a chimera which retains high affinity TSH binding and the cAMP response to TSH but loses both the cAMP response to Graves' IgG and Graves' IgG inhibition of TSH binding. Two of three IgGs from idiopathic myxedema patients which contain thyroid stimulation blocking antibodies (TSBAbs) still, however, react with this chimera. Chimeras which substitute residues 90-165 (Mc2) and 261-370 (Mc4) retain the ability to interact with TSH, Graves' IgG, and idiopathic myxedema IgG. The data thus suggest that residues 8-165 contain an epitope specific for TSAbs and that TSH receptor determinants important for the activities of TSAbs and TSH are not identical. Further, binding sites for TSBAbs in idiopathic myxedema may be different from receptor binding sites for both Graves' IgG TSAb as well as TSH and may be different in individual patients.
|
| 巻・号 |
179(1)
|
| ページ |
70-7
|
| 公開日 |
1991-8-30
|
| DOI |
10.1016/0006-291x(91)91335-a
|
| PII |
0006-291X(91)91335-A
|
| PMID |
1883391
|
| MeSH |
Base Sequence
Chimera
Cloning, Molecular
DNA / genetics
DNA / isolation & purification
Graves Disease / immunology*
Humans
Immunoglobulins / metabolism*
Kinetics
Molecular Sequence Data
Myxedema / immunology*
Oligonucleotide Probes
Receptors, LH / genetics
Receptors, LH / immunology*
Receptors, LH / metabolism
Receptors, Thyrotropin / genetics
Receptors, Thyrotropin / immunology*
Receptors, Thyrotropin / metabolism
Recombinant Proteins / immunology
Recombinant Proteins / metabolism
Restriction Mapping
Thyrotropin / metabolism
|
| IF |
2.985
|
| リソース情報 |
| 遺伝子材料 |
hTSHR (RDB06008)
Mc2b (RDB05991)
Mc1 (RDB05992)
Mc1+2 (RDB05993)
Mc1-4 (RDB05994)
rLHR (RDB05995)
Mc2a (RDB05996)
MC124 (RDB05997)
Mc1-3 (RDB05998)
Mc2+4 (RDB05999)
Mc234 (RDB06000)
Mc3 (RDB06001)
Mc2+3 (RDB06002)
Mc2 (RDB06003)
Rc2.45 (RDB06004)
Mc1b (RDB06005)
Mc1+2b (RDB06006)
Mc4 (RDB06007) |
