Reference - Detail
| RRC ID | 67379 |
|---|---|
| Author | van de Schootbrugge C, van Asten F, Nagtegaal ID, Versleijen-Jonkers YM, van Laarhoven HW, Roeffen MH, Sweep FC, Bussink J, Kaanders JH, Pruijn GJ, Boelens WC, Span PN. |
| Title | αB-crystallin expression is correlated with phospho-ERK1/2 expression in human breast cancer. |
| Journal | Int J Biol Markers |
| Abstract |
αB-crystallin is regarded as a biomarker for triple-negative and/or basal-like breast cancer. In normal breast cells, overexpression of αB-crystallin leads to neoplastic-like changes, which likely relate to enhanced expression of phosphorylated ERK1/2 (pERK1/2). In this study, we investigated whether αB-crystallin expression is correlated to pERK1/2 expression in breast cancer. In a balanced tissue microarray the expression of αB-crystallin and pERK1/2 kinase were determined immunohistochemically, together with the triple-negativity and basal-like markers CK5/6 and SMA and the signaling molecules pAKT, pmTOR, EGFR, and IGF-1R. αB-crystallin expression significantly correlated with triple negativity and basal-like markers CK5/6 and SMA (Pearson Chi-square test p=0.004, p=0.001, and p<0.001, respectively). A significant correlation was also observed with pERK1/2 expression (p=0.002). siRNA-mediated knockdown of αB-crystallin in the triple-negative breast cell line MDA-MB468 did not show an effect on pERK1/2 expression levels, indicating that lowering the level of αB-crystallin does not reduce pERK1/2 expression. Our results confirm that αB-crystallin can be used as a biomarker for triple-negative and/or basal-like breast cancer. The expression of αB-crystallin correlates with pERK1/2 expression in breast cancer tissue suggesting that therapies targeting αB-crystallin might be considered for treatment of triple-negative or basal-like breast cancer. |
| Volume | 28(4) |
| Pages | e365-70 |
| Published | 2013-12-17 |
| DOI | 10.5301/jbm.5000032 |
| PII | 1F14C83E-606C-4A9F-8F68-4631DB68746D |
| PMID | 23722303 |
| MeSH | Biomarkers, Tumor / biosynthesis Cell Line, Tumor Cohort Studies Female Gene Knockdown Techniques Humans Immunohistochemistry Mitogen-Activated Protein Kinase 1 / biosynthesis* Mitogen-Activated Protein Kinase 1 / metabolism Mitogen-Activated Protein Kinase 3 / biosynthesis* Mitogen-Activated Protein Kinase 3 / metabolism Phosphorylation Triple Negative Breast Neoplasms / enzymology Triple Negative Breast Neoplasms / metabolism* alpha-Crystallin B Chain / biosynthesis* alpha-Crystallin B Chain / genetics |
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| Total number of mentions | 0 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | anti-αB-crystallin(RCB1304) |