RRC ID 67388
Author Koh T, Yokota J, Ookawa K, Kina T, Koshimura K, Miwa S, Ariyasu T, Yamada H, Osaka M, Haga H.
Title Alternative splicing of the neurofibromatosis 1 gene correlates with growth patterns and neuroendocrine properties of human small-cell lung-carcinoma cells.
Journal Int J Cancer
Abstract Two distinct transcripts, type I and type II, of the neurofibromatosis I (NFI) gene are generated by alternative splicing in the region corresponding to the gene's GTPase-activating protein-related domain (GRD). Relative expression levels of these 2 transcripts were previously correlated to neural differentiation. Since small-cell lung carcinoma (SCLC) often exhibits neuroendocrine properties, we analyzed the type-I to type-II mRNA ratio in 15 SCLC cell lines, using reverse transcriptase and polymerase chain reaction methods. The type-I mRNA was predominant in 10 cell lines; 8 of them grew as floating aggregates in culture and had high L-dopa decarboxylase (DDC) activity. The other 5 lines predominantly expressed type-II mRNA, adhered to the culture substrate, and expressed low or undetectable levels of neural cell-adhesion molecule (NCAM) antigen and DDC activity. N2+, one of the subclones of NCI-N417 cells, exhibited a higher type-I to type-II ratio after the cells had adhered to a laminin-coated plate and had emitted neurite-like processes. These findings provide evidence that alternative splicing patterns of NFI mRNA correlate with the mechanisms that regulate the growth patterns and neuroendocrine properties of SCLC cells in vitro.
Volume 60(6)
Pages 843-7
Published 1995-3-16
DOI 10.1002/ijc.2910600620
PMID 7896456
MeSH Alternative Splicing / genetics* Carcinoma, Small Cell / enzymology Carcinoma, Small Cell / genetics* Carcinoma, Small Cell / pathology* Cell Adhesion Molecules, Neuronal / biosynthesis Cell Division / drug effects Dopa Decarboxylase / metabolism Gene Expression Regulation, Neoplastic / genetics* Genes, Neurofibromatosis 1 / genetics* Humans Laminin / pharmacology Lung Neoplasms / enzymology Lung Neoplasms / genetics* Lung Neoplasms / pathology* Tumor Cells, Cultured
IF 5.145
Human and Animal Cells Lu-134-B(RCB0467) Lu-135(RCB0468) Lu-139(RCB0469) Lu-140(RCB0470)