Reference - Detail
| RRC ID | 67388 |
|---|---|
| Author | Koh T, Yokota J, Ookawa K, Kina T, Koshimura K, Miwa S, Ariyasu T, Yamada H, Osaka M, Haga H. |
| Title | Alternative splicing of the neurofibromatosis 1 gene correlates with growth patterns and neuroendocrine properties of human small-cell lung-carcinoma cells. |
| Journal | Int J Cancer |
| Abstract |
Two distinct transcripts, type I and type II, of the neurofibromatosis I (NFI) gene are generated by alternative splicing in the region corresponding to the gene's GTPase-activating protein-related domain (GRD). Relative expression levels of these 2 transcripts were previously correlated to neural differentiation. Since small-cell lung carcinoma (SCLC) often exhibits neuroendocrine properties, we analyzed the type-I to type-II mRNA ratio in 15 SCLC cell lines, using reverse transcriptase and polymerase chain reaction methods. The type-I mRNA was predominant in 10 cell lines; 8 of them grew as floating aggregates in culture and had high L-dopa decarboxylase (DDC) activity. The other 5 lines predominantly expressed type-II mRNA, adhered to the culture substrate, and expressed low or undetectable levels of neural cell-adhesion molecule (NCAM) antigen and DDC activity. N2+, one of the subclones of NCI-N417 cells, exhibited a higher type-I to type-II ratio after the cells had adhered to a laminin-coated plate and had emitted neurite-like processes. These findings provide evidence that alternative splicing patterns of NFI mRNA correlate with the mechanisms that regulate the growth patterns and neuroendocrine properties of SCLC cells in vitro. |
| Volume | 60(6) |
| Pages | 843-7 |
| Published | 1995-3-16 |
| DOI | 10.1002/ijc.2910600620 |
| PMID | 7896456 |
| MeSH | Alternative Splicing / genetics* Carcinoma, Small Cell / enzymology Carcinoma, Small Cell / genetics* Carcinoma, Small Cell / pathology* Cell Adhesion Molecules, Neuronal / biosynthesis Cell Division / drug effects Dopa Decarboxylase / metabolism Gene Expression Regulation, Neoplastic / genetics* Genes, Neurofibromatosis 1 / genetics* Humans Laminin / pharmacology Lung Neoplasms / enzymology Lung Neoplasms / genetics* Lung Neoplasms / pathology* Tumor Cells, Cultured |
| IF | 5.145 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 4 |
| Altmetric score changes over past 6months | 3.0 |
| Resource | |
| Human and Animal Cells | Lu-134-B(RCB0467) Lu-135(RCB0468) Lu-139(RCB0469) Lu-140(RCB0470) |