| RRC ID |
67388
|
| 著者 |
Koh T, Yokota J, Ookawa K, Kina T, Koshimura K, Miwa S, Ariyasu T, Yamada H, Osaka M, Haga H.
|
| タイトル |
Alternative splicing of the neurofibromatosis 1 gene correlates with growth patterns and neuroendocrine properties of human small-cell lung-carcinoma cells.
|
| ジャーナル |
Int J Cancer
|
| Abstract |
Two distinct transcripts, type I and type II, of the neurofibromatosis I (NFI) gene are generated by alternative splicing in the region corresponding to the gene's GTPase-activating protein-related domain (GRD). Relative expression levels of these 2 transcripts were previously correlated to neural differentiation. Since small-cell lung carcinoma (SCLC) often exhibits neuroendocrine properties, we analyzed the type-I to type-II mRNA ratio in 15 SCLC cell lines, using reverse transcriptase and polymerase chain reaction methods. The type-I mRNA was predominant in 10 cell lines; 8 of them grew as floating aggregates in culture and had high L-dopa decarboxylase (DDC) activity. The other 5 lines predominantly expressed type-II mRNA, adhered to the culture substrate, and expressed low or undetectable levels of neural cell-adhesion molecule (NCAM) antigen and DDC activity. N2+, one of the subclones of NCI-N417 cells, exhibited a higher type-I to type-II ratio after the cells had adhered to a laminin-coated plate and had emitted neurite-like processes. These findings provide evidence that alternative splicing patterns of NFI mRNA correlate with the mechanisms that regulate the growth patterns and neuroendocrine properties of SCLC cells in vitro.
|
| 巻・号 |
60(6)
|
| ページ |
843-7
|
| 公開日 |
1995-3-16
|
| DOI |
10.1002/ijc.2910600620
|
| PMID |
7896456
|
| MeSH |
Alternative Splicing / genetics*
Carcinoma, Small Cell / enzymology
Carcinoma, Small Cell / genetics*
Carcinoma, Small Cell / pathology*
Cell Adhesion Molecules, Neuronal / biosynthesis
Cell Division / drug effects
Dopa Decarboxylase / metabolism
Gene Expression Regulation, Neoplastic / genetics*
Genes, Neurofibromatosis 1 / genetics*
Humans
Laminin / pharmacology
Lung Neoplasms / enzymology
Lung Neoplasms / genetics*
Lung Neoplasms / pathology*
Tumor Cells, Cultured
|
| IF |
5.145
|
| リソース情報 |
| ヒト・動物細胞 |
Lu-134-B(RCB0467)
Lu-135(RCB0468)
Lu-139(RCB0469)
Lu-140(RCB0470) |
