| RRC ID |
67395
|
| Author |
Oshima T, Takahashi K, Inada A, Yamasaki M, Yamasaki Y, Eto N.
|
| Title |
Enhanced water dispersibility and permeability through a Caco-2 cell monolayer of β-cryptoxanthin extracted from kumquats by complexation with casein.
|
| Journal |
Food Chem
|
| Abstract |
β-Cryptoxanthin (BCX) possesses potential therapeutic and health benefits. However, BCX absorption is low because of its poor aqueous solubility. In this study, a complex between BCX and casein (Cas) was prepared to improve the water dispersibility and bioavailability of BCX. BCX was recovered quantitatively from freeze-dried kumquat powder through solid-liquid extraction and saponification. The complexation significantly improved the apparent solubility of BCX under acidic and neutral conditions. A cell membrane permeation test using a Caco-2 cell monolayer was performed to evaluate the bioavailability of the BCX-Cas complex. This complex and a blank sample were digested in vitro and added to the apical side of the Caco-2 cell membrane. The quantity of BCX that permeated using the BCX-Cas complex after 24 h was 22.7 times greater than that of the blank. Thus, complexation of BCX with Cas improved dramatically the bioavailability of BCX from a kumquat extract.
|
| Volume |
360
|
| Pages |
129822
|
| Published |
2021-10-30
|
| DOI |
10.1016/j.foodchem.2021.129822
|
| PII |
S0308-8146(21)00828-1
|
| PMID |
33984564
|
| MeSH |
Beta-Cryptoxanthin / chemistry*
Caco-2 Cells
Caseins / chemistry*
Humans
Permeability
Plant Extracts / chemistry
Rutaceae / chemistry*
Solubility
Water / chemistry
|
| IF |
6.306
|
| Resource |
| Human and Animal Cells |
CACO-2(RCB0988) |
