RRC ID 6740
著者 Shimada N, Kawata T.
タイトル Evidence that noncoding RNA dutA is a multicopy suppressor of Dictyostelium discoideum STAT protein Dd-STATa.
ジャーナル Eukaryot Cell
Abstract Dd-STATa, a Dictyostelium discoideum homologue of metazoan STAT transcription factors, is necessary for culmination. We created a mutant strain with partial Dd-STATa activity and used it to screen for unlinked suppressor genes. We screened approximately 450,000 clones from a slug-stage cDNA library for their ability to rescue the culmination defect when overexpressed. There were 12 multicopy suppressors of Dd-STATa, of which 4 encoded segments of a known noncoding RNA, dutA. Expression of dutA is specific to the pstA zone, the region where Dd-STATa is activated. In suppressed strains the expression patterns of several putative Dd-STATa target genes become similar to the wild-type strain. In addition, the amount of the tyrosine-phosphorylated form of Dd-STATa is significantly increased in the suppressed strain. These results indicate that partial copies of dutA may act upstream of Dd-STATa to regulate tyrosine phosphorylation by an unknown mechanism.
巻・号 6(6)
ページ 1030-40
公開日 2007-6-1
DOI 10.1128/EC.00035-07
PII EC.00035-07
PMID 17435008
PMC PMC1951520
MeSH Animals Dictyostelium* / genetics Dictyostelium* / metabolism Genes, Protozoan* MicroRNAs / genetics MicroRNAs / metabolism Phosphorylation Protozoan Proteins / genetics Protozoan Proteins / metabolism* RNA Precursors / genetics RNA Precursors / metabolism RNA, Small Interfering / genetics RNA, Small Interfering / metabolism RNA, Untranslated / genetics RNA, Untranslated / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism Repressor Proteins / genetics Repressor Proteins / metabolism* STAT Transcription Factors / genetics STAT Transcription Factors / metabolism* Tyrosine / metabolism
IF 2.992
引用数 12
WOS 分野 MYCOLOGY MICROBIOLOGY
リソース情報
細胞性粘菌 G24894