RRC ID 67410
Author Liu H, Li C, Shen C, Yin F, Wang K, Liu Y, Zheng B, Zhang W, Hou X, Chen X, Wu J, Wang X, Zhong C, Zhang J, Shi H, Ai J, Zhao S.
Title MiR-212-3p inhibits glioblastoma cell proliferation by targeting SGK3.
Journal J Neurooncol
Abstract Glioblastoma multiforme (GBM) is the most malignant brain tumor in humans. Previous studies have demonstrated that microRNA plays important roles in the development and proliferation of GBM cells. Here we defined the mechanism by which miR-212-3p regulated the proliferation of GBM. In this study, we showed that miR-212-3p expression was significantly down-regulated and negatively correlated with serum and glucocorticoid-inducible kinase 3 (SGK3) in GBM. Either over-expression of miR-212-3p or silence of SGK3 decreased viability of GBM cells. Moreover, miR-212-3p directly bound to 3'UTR of SGK3 and inhibited its mRNA and protein expression. And over-expression of SGK3 rescued the decreased proliferation of GBM cells induced by miR-212-3p. Importantly, miR-212-3p also suppressed tumor growth in vivo. Collectively, our results demonstrated that miR-212-3p inhibited proliferation of GBM cells by directly targeting SGK3, and could potentially serve as a new therapeutic target for GBM.
Volume 122(3)
Pages 431-9
Published 2015-5-1
DOI 10.1007/s11060-015-1736-y
PMID 25720694
MeSH Animals Brain Neoplasms / pathology* Cell Line, Tumor Cell Proliferation / genetics Female Gene Expression Regulation, Neoplastic / genetics* Glioblastoma / psychology* Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Humans Linear Models Male Mice Mice, Nude MicroRNAs / genetics MicroRNAs / metabolism* Protein Serine-Threonine Kinases / genetics Protein Serine-Threonine Kinases / metabolism* RNA, Messenger / metabolism RNA, Small Interfering / metabolism Transfection Xenograft Model Antitumor Assays
IF 3.267
Human and Animal Cells T98G(RCB1954) U251(RCB0461)