| RRC ID |
67443
|
| 著者 |
Kojima C, Kameyama R, Yamada M, Ichikawa M, Waku T, Handa A, Tanaka N.
|
| タイトル |
Ovalbumin Delivery by Guanidine-Terminated Dendrimers Bearing an Amyloid-Promoting Peptide via Nanoparticle Formulation.
|
| ジャーナル |
Bioconjug Chem
|
| Abstract |
Development of protein delivery systems is important for biomedical applications such as immunotherapy. Ovalbumin (OVA) is a major component of egg whites, and is a possible cause of egg allergy. In this study, OVA was used as a model protein to develop a delivery system using guanidine-terminated dendrimers (Gdn-den) bearing an amyloid-promoting peptide derived from the helix B (hB) region of OVA (hB-Gdn-den). OVA nanoparticles (NPs) were prepared by heat treatment of OVA/hB-Gdn-den mixtures. The NP size and the surface charge were controlled by adjusting the ratio of hB-Gdn-den to OVA. The NPs were around 200 nm in diameter and stably dispersed, and their encapsulation efficiency for OVA was more than 80%. Although OVA NPs were also prepared using Gdn-den, the NPs aggregated readily. Complexation with hB-Gdn-den induced conformational changes in the OVA, and the hB peptide promoted digestion of OVA. These suggest that the hB peptide of the Gdn-den works as a possible anchor to OVA. The positively charged OVA NPs effectively associated with RAW264 cells. Thus, the amyloid-promoting Gdn-den, when mixed with OVA at a suitable molar ratio to form NPs, could act as a carrier for delivery of antigen proteins to immune cells.
|
| 巻・号 |
26(8)
|
| ページ |
1804-10
|
| 公開日 |
2015-8-19
|
| DOI |
10.1021/acs.bioconjchem.5b00325
|
| PMID |
26186179
|
| MeSH |
Amyloid / chemistry*
Animals
Cells, Cultured
Chemistry, Pharmaceutical
Dendrimers / chemistry*
Drug Delivery Systems*
Guanidine / chemistry*
Macrophages
Mice
Nanoparticles / chemistry*
Ovalbumin / administration & dosage*
Ovalbumin / metabolism
Peptide Fragments / chemistry*
|
| IF |
4.031
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
