論文 - 詳細
| RRC ID | 67443 |
|---|---|
| 著者 | Kojima C, Kameyama R, Yamada M, Ichikawa M, Waku T, Handa A, Tanaka N. |
| タイトル | Ovalbumin Delivery by Guanidine-Terminated Dendrimers Bearing an Amyloid-Promoting Peptide via Nanoparticle Formulation. |
| ジャーナル | Bioconjug Chem |
| Abstract |
Development of protein delivery systems is important for biomedical applications such as immunotherapy. Ovalbumin (OVA) is a major component of egg whites, and is a possible cause of egg allergy. In this study, OVA was used as a model protein to develop a delivery system using guanidine-terminated dendrimers (Gdn-den) bearing an amyloid-promoting peptide derived from the helix B (hB) region of OVA (hB-Gdn-den). OVA nanoparticles (NPs) were prepared by heat treatment of OVA/hB-Gdn-den mixtures. The NP size and the surface charge were controlled by adjusting the ratio of hB-Gdn-den to OVA. The NPs were around 200 nm in diameter and stably dispersed, and their encapsulation efficiency for OVA was more than 80%. Although OVA NPs were also prepared using Gdn-den, the NPs aggregated readily. Complexation with hB-Gdn-den induced conformational changes in the OVA, and the hB peptide promoted digestion of OVA. These suggest that the hB peptide of the Gdn-den works as a possible anchor to OVA. The positively charged OVA NPs effectively associated with RAW264 cells. Thus, the amyloid-promoting Gdn-den, when mixed with OVA at a suitable molar ratio to form NPs, could act as a carrier for delivery of antigen proteins to immune cells. |
| 巻・号 | 26(8) |
| ページ | 1804-10 |
| 公開日 | 2015-8-19 |
| DOI | 10.1021/acs.bioconjchem.5b00325 |
| PMID | 26186179 |
| MeSH | Amyloid / chemistry* Animals Cells, Cultured Chemistry, Pharmaceutical Dendrimers / chemistry* Drug Delivery Systems* Guanidine / chemistry* Macrophages Mice Nanoparticles / chemistry* Ovalbumin / administration & dosage* Ovalbumin / metabolism Peptide Fragments / chemistry* |
| IF | 4.031 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |