RRC ID 67449
著者 Gu J, Chan LS, Wong CK, Wong NS, Wong CK, Leung KN, Mak NK.
タイトル Effect of benzo[a]pyrene on the production of vascular endothelial growth factor by human eosinophilic leukemia EoL-1 cells.
ジャーナル J Environ Pathol Toxicol Oncol
Abstract Benzo[a]pyrene (BaP) has been shown to affect both the development and response of T and B cells in the immune system. However, the effect of BaP on other immune cells, such as eosionophils, is unknown. In this study, we investigated the effect of BaP on the production of vascular endothelial growth factor (VEGF) using an in vitro eosinophilic EoL-1 cell and human umbilical vein endothelial cell (HUVEC) co-culture system. EoL-1-conditioned medium was found to promote the growth of HUVEC in a time-dependent manner. The growth stimulating activity was due to the production of VEGF by the EoL-1 cells. The production of VEGF was correlated with the enhanced expression of the phosphorylated form of extracellular signal-regulated kinases (p-ERKs) and the upregulated expression of VEGF mRNA. Furthermore, BaP-induced expression of VEGF mRNA was reduced by the ERK inhibitor PD98059. Results from this study suggested that BaP might affect the growth of endothelial cells through the modulation of VEGF production by eosinophils.
巻・号 30(3)
ページ 241-9
公開日 2011-1-1
DOI 10.1615/jenvironpatholtoxicoloncol.v30.i3.70
PII 28d5b25a1eb31fda,0a9e98fc4bb25987
PMID 22126617
MeSH Benzo(a)pyrene / pharmacology* Carcinogens / pharmacology Cell Line Coculture Techniques Endothelium, Vascular / cytology Endothelium, Vascular / drug effects Endothelium, Vascular / metabolism* Enzyme Inhibitors / pharmacology Eosinophils / drug effects Eosinophils / metabolism* Eosinophils / pathology Extracellular Signal-Regulated MAP Kinases / antagonists & inhibitors Extracellular Signal-Regulated MAP Kinases / metabolism Flavonoids / pharmacology Humans Hypereosinophilic Syndrome / pathology* RNA, Messenger / metabolism Vascular Endothelial Growth Factor A / metabolism*
リソース情報
ヒト・動物細胞 EoL-1 cell(RCB0641)