Reference - Detail
RRC ID | 67468 |
---|---|
Author | Iguchi Y, Ishihara S, Uchida Y, Tajima K, Mizutani T, Kawabata K, Haga H. |
Title | Filamin B Enhances the Invasiveness of Cancer Cells into 3D Collagen Matrices. |
Journal | Cell Struct Funct |
Abstract |
Numerous types of cancer cells migrate into extracellular tissues. This phenomenon is termed invasion, and is associated with poor prognosis in cancer patients. In this study, we demonstrated that filamin B (FLNb), an actin-binding protein, is highly expressed in cancer cell lines that exhibit high invasiveness, with a spindle morphology, into 3D collagen matrices. In addition, we determined that knockdown of FLNb in invasive cancer cells converts cell morphology from spindle-shaped, which is associated with high invasiveness, to round-shaped with low invasiveness. Furthermore, di-phosphorylation of myosin regulatory light chain (MRLC) and phosphorylation of focal adhesion kinase (FAK) are inhibited in FLNb-knockdown cancer cells. These results suggest that FLNb enhances invasion of cancer cells through phosphorylation of MRLC and FAK. Therefore, FLNb may be a new therapeutic target for invasive cancers. |
Volume | 40(2) |
Pages | 61-7 |
Published | 2015-1-1 |
DOI | 10.1247/csf.15001 |
PMID | 25925610 |
MeSH | Cell Adhesion Cell Culture Techniques Cell Line, Tumor Cell Movement Collagen / chemistry Epithelial Cells / metabolism* Epithelial Cells / pathology Fibroblasts / metabolism* Fibroblasts / pathology Filamins / antagonists & inhibitors Filamins / genetics* Filamins / metabolism Focal Adhesion Kinase 1 / genetics* Focal Adhesion Kinase 1 / metabolism Gene Expression Regulation, Neoplastic* Humans Myosin Light Chains / genetics* Myosin Light Chains / metabolism Phosphorylation RNA, Small Interfering / genetics RNA, Small Interfering / metabolism Signal Transduction |
IF | 2.429 |
Resource | |
Human and Animal Cells | A549 |