Reference - Detail
| RRC ID | 67480 |
|---|---|
| Author | Obitsu S, Ahmed N, Nishitsuji H, Hasegawa A, Nakahama K, Morita I, Nishigaki K, Hayashi T, Masuda T, Kannagi M. |
| Title | Potential enhancement of osteoclastogenesis by severe acute respiratory syndrome coronavirus 3a/X1 protein. |
| Journal | Arch Virol |
| Abstract |
Severe acute respiratory syndrome coronavirus (SARS-CoV) causes a lung disease with high mortality. In addition, osteonecrosis and bone abnormalities with reduced bone density have been observed in patients following recovery from SARS, which were partly but not entirely explained by the short-term use of steroids. Here, we demonstrate that human monocytes, potential precursors of osteoclasts, partly express angiotensin converting enzyme 2 (ACE2), a cellular receptor of SARS-CoV, and that expression of an accessory protein of SARS-CoV, 3a/X1, in murine macrophage cell line RAW264.7 cells, enhanced NF-kappaB activity and differentiation into osteoclast-like cells in the presence of receptor activator of NF-kappaB ligand (RANKL). Furthermore, human epithelial A549 cells expressed ACE2, and expression of 3a/X1 in these cells up-regulated TNF-alpha, which is known to accelerate osteoclastogenesis. 3a/X1 also enhanced RANKL expression in mouse stromal ST2 cells. These findings indicate that SARS-CoV 3a/X1 might promote osteoclastogenesis by direct and indirect mechanisms. |
| Volume | 154(9) |
| Pages | 1457-64 |
| Published | 2009-1-1 |
| DOI | 10.1007/s00705-009-0472-z |
| PMID | 19685004 |
| PMC | PMC7086770 |
| MeSH | Angiotensin-Converting Enzyme 2 Animals Bone Marrow Cells / cytology Bone Resorption / virology* Cell Line Epithelial Cells / metabolism Humans Lung / metabolism Macrophages / metabolism Mice Monocytes / metabolism Osteoblasts / metabolism* Peptidyl-Dipeptidase A / genetics Peptidyl-Dipeptidase A / metabolism RANK Ligand / metabolism Severe Acute Respiratory Syndrome / metabolism Severe Acute Respiratory Syndrome / pathology* Severe Acute Respiratory Syndrome / virology Severe acute respiratory syndrome-related coronavirus* Stromal Cells / metabolism Tumor Necrosis Factor-alpha / metabolism Up-Regulation Viral Structural Proteins / metabolism* |
| IF | 2.243 |
| Altmetric score |
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | ST2(RCB0224) |