論文 - 詳細
| RRC ID | 67496 |
|---|---|
| 著者 | Su BC, Hung GY, Tu YC, Yeh WC, Lin MC, Chen JY. |
| タイトル | Marine Antimicrobial Peptide TP4 Exerts Anticancer Effects on Human Synovial Sarcoma Cells via Calcium Overload, Reactive Oxygen Species Production and Mitochondrial Hyperpolarization. |
| ジャーナル | Mar Drugs |
| Abstract |
Synovial sarcoma is a rare but aggressive soft-tissue sarcoma associated with translocation t(X;18). Metastasis occurs in approximately 50% of all patients, and curative outcomes are difficult to achieve in this group. Since the efficacies of current therapeutic approaches for metastatic synovial sarcoma remain limited, new therapeutic agents are urgently needed. Tilapia piscidin 4 (TP4), a marine antimicrobial peptide, is known to exhibit multiple biological functions, including anti-bacterial, wound-healing, immunomodulatory, and anticancer activities. In the present study, we assessed the anticancer activity of TP4 in human synovial sarcoma cells and determined the underlying mechanisms. We first demonstrated that TP4 can induce necrotic cell death in human synovial sarcoma AsKa-SS and SW982 cells lines. In addition, we saw that TP4 initiates reactive oxygen species (ROS) production and downregulates antioxidant proteins, such as uncoupling protein-2, superoxide dismutase (SOD)-1, and SOD-2. Moreover, TP4-induced mitochondrial hyperpolarization is followed by elevation of mitochondrial ROS. Calcium overload is also triggered by TP4, and cell death can be attenuated by a necrosis inhibitor, ROS scavenger or calcium chelator. In our experiments, TP4 displayed strong anticancer activity in human synovial sarcoma cells by disrupting oxidative status, promoting mitochondrial hyperpolarization and causing calcium overload. |
| 巻・号 | 19(2) |
| 公開日 | 2021-2-5 |
| DOI | 10.3390/md19020093 |
| PII | md19020093 |
| PMID | 33562681 |
| PMC | PMC7915578 |
| MeSH | Animals Antineoplastic Agents / pharmacology* Calcium / metabolism* Cell Line, Tumor Cell Survival / drug effects Fish Proteins / pharmacology* Humans Mitochondria / drug effects* Mitochondria / physiology Pore Forming Cytotoxic Proteins / pharmacology* Reactive Oxygen Species / metabolism* Sarcoma, Synovial / drug therapy* Sarcoma, Synovial / metabolism Tilapia / metabolism* |
| IF | 4.073 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Aska-SS(RCB3576) |