RRC ID 6752
著者 Hirata K, Amagai A, Chae SC, Hirose S, Maeda Y.
タイトル Involvements of a novel protein, DIA2, in cAMP signaling and spore differentiation during Dictyostelium development.
ジャーナル Differentiation
Abstract The novel gene dia2 (differentiation-associated gene 2) was originally isolated as a gene expressed specifically in response to initial differentiation of Dictyostelium discoideum Ax-2 cells. Using dia2(AS) cells in which the dia2 expression was inactivated by the antisense RNA method, DIA2 protein was found to be required for cAMP signaling during cell aggregation. During late development, the DIA2 protein changed its location from the endoplasmic reticulum (ER) to prespore-specific vacuoles (PSVs) that are specifically present in prespore cells of the slug. In differentiating prestalk cells, however, DIA2 was found to be nearly lost from the cells. Importantly, exocytosis of PSVs from prespore cells and the subsequent spore differentiation were almost completely impaired in dia2(AS) cells. In addition, spore induction by externally applied 8-bromo cAMP was significantly suppressed in dia2(AS) cells. Taken together, these results strongly suggested that DIA2 might be closely involved in cAMP signaling and spore differentiation as well as in the initiation of differentiation during Dictyostelium development.
巻・号 76(3)
ページ 310-22
公開日 2008-3-1
DOI 10.1111/j.1432-0436.2007.00217.x
PII S0301-4681(09)60075-X
PMID 17825085
MeSH Amino Acid Sequence Animals Base Sequence Blotting, Western Cyclic AMP / metabolism* DNA Primers Dictyostelium / growth & development* Dictyostelium / physiology Exocytosis Molecular Sequence Data Protozoan Proteins / genetics Protozoan Proteins / physiology* Signal Transduction* Spores, Fungal*
IF 2.392
引用数 4
WOS 分野 DEVELOPMENTAL BIOLOGY CELL BIOLOGY
リソース情報
細胞性粘菌 G02390 S90293