RRC ID 6754
Author Bennett N, Letourneur F, Ragno M, Louwagie M.
Title Sorting of the v-SNARE VAMP7 in Dictyostelium discoideum: a role for more than one Adaptor Protein (AP) complex.
Journal Exp. Cell Res.
Abstract Soluble N-ethylmaleimide-sensitive-factor Attachment protein Receptors (SNAREs) participate in the specificity of membrane fusions in the cell. The mechanisms of specific SNARE sorting are still however poorly documented. We investigated the possible role of Adaptor Protein (AP) complexes in sorting of the Dictyostelium discoideum v-SNARE VAMP7. In live cells, GFP-VAMP7 is observed in the membrane of endocytic compartments. It is also observed in the plasma membrane of a small proportion of the cells. Mutation of a potential dileucine motif dramatically increases the proportion of cells with GFP-VAMP7 in their plasma membrane, strongly supporting the participation of an AP complex in VAMP7 sorting to the endocytic pathway. A partial increase occurs in knockout cells for the medium subunits of AP-2 and AP-3 complexes, indicating a role for both AP-2 and AP-3. VAMP7, as well as its t-SNAREs partners syntaxin 8 and Vti1, are co-immunoprecipitated with each of the medium subunits of the AP-1, AP-2, AP-3 and AP-4 complexes. This result supports the conclusion that VAMP7 directly interacts with both AP-2 and AP-3. It also raises the hypothesis of an interaction with AP-1 and AP-4. GFP-VAMP7 is retrieved from the endocytic pathway at and/or before the late post-lysosomal stage through an AP-independent mechanism.
Volume 314(15)
Pages 2822-33
Published 2008-9-10
DOI 10.1016/j.yexcr.2008.06.019
PII S0014-4827(08)00244-9
PMID 18634783
MeSH Adaptor Protein Complex 2 / metabolism Adaptor Proteins, Vesicular Transport / metabolism* Animals Cell Membrane / metabolism Cell Membrane / ultrastructure Dictyostelium / metabolism* Endocytosis / physiology* Green Fluorescent Proteins / genetics Intracellular Membranes / metabolism Intracellular Membranes / ultrastructure Macromolecular Substances / metabolism Protein Transport / physiology Protozoan Proteins / metabolism R-SNARE Proteins / metabolism* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism SNARE Proteins / metabolism* Signal Transduction / physiology Transport Vesicles / metabolism* Transport Vesicles / ultrastructure
IF 3.309
Times Cited 9
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