論文 - 詳細
| RRC ID | 67542 |
|---|---|
| 著者 | Saito K, Kobayashi M, Nagashio R, Ryuge S, Katono K, Nakashima H, Tsuchiya B, Jiang SX, Saegusa M, Satoh Y, Masuda N, Sato Y. |
| タイトル | S100A16 is a Prognostic Marker for Lung Adenocarcinomas. |
| ジャーナル | Asian Pac J Cancer Prev |
| Abstract |
BACKGROUND:Many functional molecules controlling diverse cellular function are included in low-molecular weight proteins and peptides. MATERIALS AND METHODS:To identify proteins controlling function in lung adenocarcinomas (AC), we performed two-dimensional gel electrophoresis employing tricine-SDS polyacrylamide in the second dimension (tricine 2-DE). This system was able to detect proteins under 1 kDa even with post- translational modifications. To confirm the utility of detected proteins as novel tumor markers for AC, we performed immunohistochemical analysis using 170 formalin-fixed and paraffin-embedded lung AC tissues. RESULTS:Tricine 2-DE revealed that five proteins including S100A16 were overexpressed in lung AC-derived cells compared with lung squamous cell carcinoma, small cell carcinoma, and large cell neuroendocrine carcinoma- derived cells. Immunohistochemically, S100A16 showed various subcellular localization in lung cancer tissues and a membranous staining status was correlated with the T-factor (P=0.0008), pathological stage (P=0.0015), differentiation extent (P=0.0001), lymphatic invasion (P=0.0007), vascular invasion (P=0.0001), pleural invasion (P=0.0087), and gender (P=0.039), but not with the age or smoking history. More importantly, membranous staining of S100A16 was significantly correlated with a poorer overall survival of either stage I (P=0.0088) or stage II / III (P=0.0003) lung AC patients, and multivariate analysis confirmed that membranous expression of S100A16 was an independent adverse prognostic indicator (P=0.0001). CONCLUSIONS:The present results suggest that S100A16 protein is a novel prognostic marker for lung AC. |
| 巻・号 | 16(16) |
| ページ | 7039-44 |
| 公開日 | 2015-1-1 |
| DOI | 10.7314/apjcp.2015.16.16.7039 |
| PMID | 26514487 |
| MeSH | Adenocarcinoma / chemistry* Adenocarcinoma / metabolism Adenocarcinoma / pathology* Aged Biomarkers, Tumor / analysis Blood Vessels / pathology Carcinoma, Neuroendocrine / metabolism Carcinoma, Squamous Cell / metabolism Cell Line, Tumor Cell Membrane / chemistry* Female Humans Immunohistochemistry Lung Neoplasms / chemistry* Lung Neoplasms / metabolism Lung Neoplasms / pathology* Male Middle Aged Neoplasm Grading Neoplasm Invasiveness Neoplasm Staging Pleura / pathology Prognosis S100 Proteins / analysis* S100 Proteins / metabolism Sex Factors Small Cell Lung Carcinoma / metabolism Survival Rate |
| リソース情報 | |
| ヒト・動物細胞 | LC-2/ad(RCB0440) RERF-LC-AI(RCB0444) |