RRC ID 67588
著者 Ashizawa N, Shimizu H, Shoda K, Furuya S, Akaike H, Hosomura N, Kawaguchi Y, Amemiya H, Kawaida H, Sudo M, Inoue S, Kono H, Katsurahara K, Shiozaki A, Ichikawa D.
タイトル NADPH oxidase 5 has a crucial role in cellular motility of colon cancer cells.
ジャーナル Int J Oncol
Abstract NADPH oxidases (NOXs) are a family of transmembrane proteins that generate reactive oxygen species. It was previously reported that patients with colon cancer who had high NOX5 expression had poor prognosis. However, no studies have investigated the cellular functions of NOX5 in colon cancer. The present study aimed to clarify the relationship between NOX5 and cancer development using an in vitro model. Reverse transcription‑quantitative PCR was performed to determine the NOX5 expression levels of colon cancer cell lines. NOX5‑knockdown experiments were conducted, and the effect on cell proliferation, migration, and invasion were analyzed. In addition, mRNA microarray was conducted to assess changes in gene profile. NOX5 mRNA expression was high in HCT116 cells and moderate in SW48 cells. NOX5 knockdown significantly inhibited cell migration and invasion in both HCT116 and SW48 cells; however, NOX5 knockdown reduced cell proliferation in only HCT116 cells. mRNA microarrays revealed a strong relationship between NOX5 expression levels and integrin‑linked kinase signaling pathways. The NOX5 expression in colon cancer cells affected cancer progression, especially cell motility. NOX5 may be a novel therapeutic target for the future development of treatments for colon cancer.
巻・号 59(2)
公開日 2021-8-1
DOI 10.3892/ijo.2021.5243
PII 63
PMID 34278462
MeSH Aged Cell Line, Tumor Cell Movement Cell Proliferation Colonic Neoplasms / genetics* Colonic Neoplasms / metabolism Disease Progression Gene Expression Profiling Gene Expression Regulation, Neoplastic HCT116 Cells Humans Male NADPH Oxidase 5 / genetics* NADPH Oxidase 5 / metabolism* Oligonucleotide Array Sequence Analysis Signal Transduction Up-Regulation*
IF 3.899
リソース情報
ヒト・動物細胞 HCT116(RCB2979)