RRC ID 67591
Author Mineo S, Takahashi N, Yamada-Hara M, Tsuzuno T, Aoki-Nonaka Y, Tabeta K.
Title Rice bran-derived protein fractions enhance sulforaphane-induced anti-oxidative activity in gingival epithelial cells.
Journal Arch Oral Biol
Abstract OBJECTIVE:Food-derived bioactive peptides have been reported to exhibit various beneficial effects, including anti-microbial, anti-inflammatory, and anti-oxidant properties. Oxidative stress has been implicated in the development of several inflammatory diseases such as periodontal disease. However, the anti-oxidative effect of food-derived bioactive peptides in gingival epithelial cells (GECs) is unknown. Therefore, we examined the bioactivity of the peptides in GECs.
DESIGN:Food-derived peptide fractionations derived from rice bran, rice endosperm, corn, and soy were screened for anti-oxidative effects using anti-oxidant response element (ARE)-luciferase-transfected HEK 293 cells. The induction of anti-oxidation-related genes and proteins in GECs by the fractions were examined by quantitative PCR and Western blotting, respectively. Then, the fraction-mediated anti-oxidative effects were examined by measuring intracellular reactive oxygen species (ROS) levels using flow cytometry. Furthermore, the anti-oxidative response-related cellular signaling pathways were analyzed via Western blotting.
RESULTS:Although treatment with the food-derived peptides alone did not activate anti-oxidative responses, co-treatment with sulforaphane (SFN; a potent anti-oxidant) and certain food-derived peptides enhanced anti-oxidative responses in ARE-luciferase-transfected HEK 293 cells. The fractions augmented heme oxygenase-1 mRNA and protein expression in GECs. The percentage of ROS-positive cells was significantly decreased by co-treatment with SFN and peptide fractions derived from rice bran. Furthermore, the involvement of both nuclear factor erythroid 2-related factor 2 (Nrf2) and extracellular signal-regulated kinase (ERK) in the enhancement of anti-oxidative responses was demonstrated by Western blotting.
CONCLUSIONS:Peptides derived from rice bran enhances SFN-induced anti-oxidative responses in GECs through ERK-Nrf2-ARE signaling.
Volume 129
Pages 105215
Published 2021-9-1
DOI 10.1016/j.archoralbio.2021.105215
PII S0003-9969(21)00178-3
PMID 34325345
MeSH Antioxidants / pharmacology Epithelial Cells / metabolism HEK293 Cells Heme Oxygenase-1 Humans Isothiocyanates / pharmacology NF-E2-Related Factor 2 / metabolism Oryza* Oxidative Stress Reactive Oxygen Species Sulfoxides
IF 1.931
Human and Animal Cells Ca9-22(RCB1976)