論文 - 詳細
| RRC ID | 67592 |
|---|---|
| 著者 | Cheng TY, Lin YJ, Saburi W, Vieths S, Scheurer S, Schülke S, Toda M. |
| タイトル | β-(1→4)-Mannobiose Acts as an Immunostimulatory Molecule in Murine Dendritic Cells by Binding the TLR4/MD-2 Complex. |
| ジャーナル | Cells |
| Abstract |
Some β-mannans, including those in coffee bean and soy, contain a mannose backbone with β-(1→4) bonds. Such mannooligosaccharides could have immunological functions involving direct interaction with immune cells, in addition to acting as prebiotics. This study aimed at assessing the immunological function of mannooligosaccharides with β-(1→4) bond, and elucidating their mechanism of action using bone marrow-derived murine dendritic cells (BMDCs). When BMDCs were stimulated with the mannooligosaccharides, only β-Man-(1→4)-Man significantly induced production of cytokines that included IL-6, IL-10, TNF-α, and IFN-β, and enhanced CD4+ T-cell stimulatory capacity. Use of putative receptor inhibitors revealed the binding of β-Man-(1→4)-Man to TLR4/MD2 complex and involvement with the complement C3a receptor (C3aR) for BMDC activation. Interestingly, β-Man-(1→4)-Man prolonged the production of pro-inflammatory cytokines (IL-6 and TNF-α), but not of the IL-10 anti-inflammatory cytokine during extended culture of BMDCs, associated with high glucose consumption. The results suggest that β-Man-(1→4)-Man is an immunostimulatory molecule, and that the promotion of glycolysis could be involved in the production of pro-inflammatory cytokine in β-Man-(1→4)-Man-stimulated BMDCs. This study could contribute to development of immune-boosting functional foods and a novel vaccine adjuvant. |
| 巻・号 | 10(7) |
| 公開日 | 2021-7-14 |
| DOI | 10.3390/cells10071774 |
| PII | cells10071774 |
| PMID | 34359943 |
| PMC | PMC8305851 |
| MeSH | Animals Cell Differentiation / drug effects Cytokines / metabolism Dendritic Cells / drug effects* Dendritic Cells / immunology Female Lymphocyte Activation / drug effects Mannans / metabolism Mannans / pharmacology* Mice T-Lymphocytes / drug effects* T-Lymphocytes / immunology Toll-Like Receptor 4 / metabolism* |
| IF | 4.366 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |