論文 - 詳細
| RRC ID | 6764 |
|---|---|
| 著者 | Ikeda T, Saito T, Ushita M, Yano F, Kan A, Itaka K, Moro T, Nakamura K, Kawaguchi H, Chung UI. |
| タイトル | Identification and characterization of the human SOX6 promoter. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
The present study attempted to identify and characterize the embryonic promoter of Sox6, a determinant regulator of chondrogenic differentiation. A common transcription start region for human and mouse Sox6 was initially identified, which contained a highly conserved sequence, A-box. Tandem repeats of A-box had a strong transcriptional activity both at the basal level and in response to Sox9. Cells carrying the 4xA-box-DsRed2 reporter fluoresced only upon chondrogenic differentiation. The 46-bp core enhancer region (CES6) was then identified in the 3' half of A-box, within which a C/EBP-binding motif was identified. Overexpressed C/EBPbeta activated the Sox6 promoter, and mutant 4xCES6 constructs lacking the C/EBP motif lost their basal activity. CES6 and nuclear extracts formed a specific complex, which was supershifted by anti-C/EBPbeta antibody, and in vitro translated C/EBPbeta specifically bound to CES6. Thus, we successfully identified the Sox6 promoter and its core enhancer and characterized the interactions with regulatory transcription factors. |
| 巻・号 | 357(2) |
| ページ | 383-90 |
| 公開日 | 2007-6-1 |
| DOI | 10.1016/j.bbrc.2007.03.133 |
| PII | S0006-291X(07)00625-0 |
| PMID | 17433257 |
| MeSH | Animals Base Sequence Chondrogenesis / genetics* DNA-Binding Proteins / genetics* High Mobility Group Proteins / genetics* Humans Mice Molecular Sequence Data Promoter Regions, Genetic / genetics* Regulatory Sequences, Nucleic Acid / genetics* SOXD Transcription Factors Transcription Factors / genetics* |
| IF | 2.985 |
| 引用数 | 10 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HuH-7(RCB1366) |