| RRC ID |
67657
|
| 著者 |
Barnor JS, Habu Y, Yamamoto N, Miyano-Kurosaki N, Ishikawa K, Yamamoto N, Takaku H.
|
| タイトル |
Inhibition of HIV-1 replication by long-term treatment with a chimeric RNA containing shRNA and TAR decoy RNA.
|
| ジャーナル |
Antiviral Res
|
| Abstract |
Combinatorial therapies for the treatment of HIV-1 infection are effective for reducing patient viral loads and slowing the progression to AIDS. Our strategy was based on an anti-HIV-1 shRNA vector system in which HIV-1 vif-shRNA was fused to a decoy TAR RNA (mini-TAR RNA) to generate vif-shRNA-decoy TAR RNA under the control of the human U6 Pol III promoter. Upon expression in human cells, the RNA molecule was cleaved into its component parts, which inhibited HIV-1 replication in a synergistic manner. This chimeric RNA expressed a dual RNA moiety and greatly enhanced the inhibition of HIV-1 replication under the production of resistant virus by short interference RNA (siRNA) in long-term culture assays. We suggest that this technique provides a practical basis for the application of siRNA-based gene therapy in the treatment of HIV/AIDS.
|
| 巻・号 |
83(2)
|
| ページ |
156-64
|
| 公開日 |
2009-8-1
|
| DOI |
10.1016/j.antiviral.2009.04.008
|
| PII |
S0166-3542(09)00317-9
|
| PMID |
19397929
|
| MeSH |
Anti-HIV Agents / pharmacology*
Cells, Cultured
HIV Long Terminal Repeat / drug effects
HIV-1 / drug effects*
HIV-1 / genetics
Humans
Leukocytes, Mononuclear / virology
RNA Interference
RNA, Small Interfering / genetics
RNA, Small Interfering / pharmacology*
Virus Replication / drug effects*
vif Gene Products, Human Immunodeficiency Virus / antagonists & inhibitors
vif Gene Products, Human Immunodeficiency Virus / genetics
|
| IF |
4.101
|
| リソース情報 |
| 遺伝子材料 |
CS-CDF-CG-PRE (RDB04379) |
