Reference - Detail
| RRC ID | 6768 |
|---|---|
| Author | Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S. |
| Title | Induction of pluripotent stem cells from adult human fibroblasts by defined factors. |
| Journal | Cell |
| Abstract |
Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts. |
| Volume | 131(5) |
| Pages | 861-72 |
| Published | 2007-11-30 |
| DOI | 10.1016/j.cell.2007.11.019 |
| PII | S0092-8674(07)01471-7 |
| PMID | 18035408 |
| MeSH | Adult Aged Animals Biomarkers / metabolism Cell Differentiation / genetics Cells, Cultured Cellular Reprogramming* DNA-Binding Proteins / genetics* DNA-Binding Proteins / metabolism Embryo, Mammalian Female Fibroblasts / metabolism Fibroblasts / physiology* Genes, myc* HMGB Proteins / genetics* HMGB Proteins / metabolism Humans Kruppel-Like Factor 4 Kruppel-Like Transcription Factors / genetics* Kruppel-Like Transcription Factors / metabolism Male Mice Mice, Inbred ICR Myocytes, Cardiac / physiology Neurons / physiology Octamer Transcription Factor-3 / genetics* Octamer Transcription Factor-3 / metabolism Pluripotent Stem Cells / metabolism Pluripotent Stem Cells / physiology* SOXB1 Transcription Factors Telomerase / metabolism Teratoma / pathology Transcription Factors / genetics* Transcription Factors / metabolism Transduction, Genetic* |
| IF | 38.637 |
| Times Cited | 11315 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Resource | |
| Human and Animal Cells | MC3T3-G2/PA6(RCB1127) |