論文 - 詳細
RRC ID | 67690 |
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著者 | Su S, Phua SC, DeRose R, Chiba S, Narita K, Kalugin PN, Katada T, Kontani K, Takeda S, Inoue T. |
タイトル | Genetically encoded calcium indicator illuminates calcium dynamics in primary cilia. |
ジャーナル | Nat Methods |
Abstract |
Visualization of signal transduction in live primary cilia constitutes a technical challenge owing to the organelle's submicrometer dimensions and close proximity to the cell body. Using a genetically encoded calcium indicator targeted to primary cilia, we visualized calcium signaling in cilia of mouse fibroblasts and kidney cells upon chemical or mechanical stimulation with high specificity, high sensitivity and wide dynamic range. |
巻・号 | 10(11) |
ページ | 1105-7 |
公開日 | 2013-11-1 |
DOI | 10.1038/nmeth.2647 |
PII | nmeth.2647 |
PMID | 24056873 |
PMC | PMC3860264 |
MeSH | Animals Calcium Signaling / genetics* Cilia / metabolism* Mice Signal Transduction |
IF | 30.822 |
リソース情報 | |
遺伝子材料 | CSII-CMV-MCS-IRES2-Bsd (RDB04385) CS-CDF-CG-PRE (RDB04379) |