論文 - 詳細
| RRC ID | 6772 |
|---|---|
| 著者 | Hirata H, Kawamata S, Murakami Y, Inoue K, Nagahashi A, Tosaka M, Yoshimura N, Miyamoto Y, Iwasaki H, Asahara T, Sawa Y. |
| タイトル | Coexpression of platelet-derived growth factor receptor alpha and fetal liver kinase 1 enhances cardiogenic potential in embryonic stem cell differentiation in vitro. |
| ジャーナル | J Biosci Bioeng |
| Abstract |
Nascent mesodermal cells derived from EB5 embryonic stem (ES) cells were sorted in terms of cardiogenic potential on the basis of their expression levels of platelet-derived growth factor receptor alpha (PDGFRalpha) and fetal liver kinase 1 (Flk-1). The sorted cells were cocultured with OP9 stromal cells to induce terminal differentiation into contractile cardiac colonies. A significant number of cardiac colonies were found in the Flk-1+/PDGFRalpha+ fraction. The enrichment double-positive fraction produced approximately fivefold more cardiac colonies than the Flk-1+/PDGFRalpha- fraction and 10-fold more than the Flk-1-/PDGFRalpha+ fraction. To investigate the involvement of these markers in embryonic cardiogenesis, the cells that disseminated from the E7.5-7.75 embryos were fractionated and seeded on OP9 cells. The cardiogenic potential was markedly enhanced in the Flk-1+/PDGFRalpha+ fraction. These results suggest that some of the precursor cells coexpressing these markers are selectively involved in cardiogenic events, and that the identification of ES-cell-derived precursors with these markers will contribute to the effective production of cardiomyocytes for cell therapies. |
| 巻・号 | 103(5) |
| ページ | 412-9 |
| 公開日 | 2007-5-1 |
| DOI | 10.1263/jbb.103.412 |
| PII | S1389-1723(07)70081-6 |
| PMID | 17609155 |
| MeSH | Animals Biomarkers / metabolism Cell Differentiation Cell Line Embryonic Development / physiology* Embryonic Stem Cells / cytology* Embryonic Stem Cells / physiology* Gene Expression / physiology Mice Mice, Inbred ICR Myocardial Contraction / physiology Myocytes, Cardiac / cytology* Myocytes, Cardiac / physiology* Receptor, Platelet-Derived Growth Factor alpha / metabolism* Vascular Endothelial Growth Factor Receptor-2 / metabolism* |
| IF | 2.366 |
| 引用数 | 25 |
| WOS 分野 | FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | OP9(RCB1124) |