論文 - 詳細
| RRC ID | 67723 |
|---|---|
| 著者 | Higuchi A, Wang CT, Ling QD, Lee HH, Kumar SS, Chang Y, Alarfaj AA, Munusamy MA, Hsu ST, Wu GJ, Umezawa A. |
| タイトル | A hybrid-membrane migration method to isolate high-purity adipose-derived stem cells from fat tissues. |
| ジャーナル | Sci Rep |
| Abstract |
Human adipose-derived stem cells (hADSCs) exhibit heterogeneous characteristics, indicating various genotypes and differentiation abilities. The isolated hADSCs can possess different purity levels and divergent properties depending on the purification methods used. We developed a hybrid-membrane migration method that purifies hADSCs from a fat tissue solution with extremely high purity and pluripotency. A primary fat-tissue solution was permeated through the porous membranes with a pore size from 8 to 25 μm, and the membranes were incubated in cell culture medium for 15-18 days. The hADSCs that migrated from the membranes contained an extremely high percentage (e.g., >98%) of cells positive for mesenchymal stem cell markers and showed almost one order of magnitude higher expression of some pluripotency genes (Oct4, Sox2, Klf4 and Nanog) compared with cells isolated using the conventional culture method. |
| 巻・号 | 5 |
| ページ | 10217 |
| 公開日 | 2015-5-13 |
| DOI | 10.1038/srep10217 |
| PII | srep10217 |
| PMID | 25970301 |
| PMC | PMC4429558 |
| MeSH | Adipose Tissue / cytology* Adult Stem Cells / cytology* Adult Stem Cells / metabolism Antigens, Surface / metabolism Biomarkers Cell Separation / methods* Gene Expression Profiling Humans Immunophenotyping Kruppel-Like Factor 4 |
| IF | 3.998 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 454E2(HPS0077) |