論文 - 詳細
| RRC ID | 67759 |
|---|---|
| 著者 | Osawa Y, Murata K, Usui M, Kuba Y, Le HT, Mikami N, Nakagawa T, Daitoku Y, Kato K, Shawki HH, Ikeda Y, Kuno A, Morimoto K, Tanimoto Y, Dinh TTH, Yagami KI, Ema M, Yoshida S, Takahashi S, Mizuno S, Sugiyama F. |
| タイトル | EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice. |
| ジャーナル | Elife |
| Abstract |
The male germ cells must adopt the correct morphology at each differentiation stage for proper spermatogenesis. The spermatogonia regulates its differentiation state by its own migration. The male germ cells differentiate and mature with the formation of syncytia, failure of forming the appropriate syncytia results in the arrest at the spermatocyte stage. However, the detailed molecular mechanisms of male germ cell morphological regulation are unknown. Here, we found that EXOC1, a member of the Exocyst complex, is important for the pseudopod formation of spermatogonia and spermatocyte syncytia in mice. EXOC1 contributes to the pseudopod formation of spermatogonia by inactivating the Rho family small GTPase Rac1 and also functions in the spermatocyte syncytia with the SNARE proteins STX2 and SNAP23. Since EXOC1 is known to bind to several cell morphogenesis factors, this study is expected to be the starting point for the discovery of many morphological regulators of male germ cells. |
| 巻・号 | 10 |
| 公開日 | 2021-5-11 |
| DOI | 10.7554/eLife.59759 |
| PII | 59759 |
| PMID | 33973520 |
| PMC | PMC8112867 |
| MeSH | Animals Cell Differentiation Gene Expression Regulation, Developmental Giant Cells Male Mice Mice, Inbred C57BL Spermatocytes / physiology* Spermatogenesis / genetics* Spermatogonia / cytology Spermatogonia / physiology* Vesicular Transport Proteins / genetics* Vesicular Transport Proteins / metabolism |
| IF | 7.08 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | T7-NLS hCas9-pA (RDB13130) pT7-3xFLAG-NLS-Flpe-polyA plasmid (RDB16011) |