論文 - 詳細
| RRC ID | 6777 |
|---|---|
| 著者 | Mizuno Y, Kurochkin IV, Herberth M, Okazaki Y, Schönbach C. |
| タイトル | Predicted mouse peroxisome-targeted proteins and their actual subcellular locations. |
| ジャーナル | BMC Bioinformatics |
| Abstract |
BACKGROUND:The import of most intraperoxisomal proteins is mediated by peroxisome targeting signals at their C-termini (PTS1) or N-terminal regions (PTS2). Both signals have been integrated in subcellular location prediction programs. However their present performance, particularly of PTS2-targeting did not seem fitting for large-scale screening of sequences. RESULTS:We modified an earlier reported PTS1 screening method to identify PTS2-containing mouse candidates using a combination of computational and manual annotation. For rapid confirmation of five new PTS2- and two previously identified PTS1-containing candidates we developed the new cell line CHO-perRed which stably expresses the peroxisomal marker dsRed-PTS1. Using CHO-perRed we confirmed the peroxisomal localization of PTS1-targeted candidate Zadh2. Preliminary characterization of Zadh2 expression suggested non-PPARalpha mediated activation. Notably, none of the PTS2 candidates located to peroxisomes. CONCLUSION:In a few cases the PTS may oscillate from "silent" to "functional" depending on its surface accessibility indicating the potential for context-dependent conditional subcellular sorting. Overall, PTS2-targeting predictions are unlikely to improve without generation and integration of new experimental data from location proteomics, protein structures and quantitative Pex7 PTS2 peptide binding assays. |
| 巻・号 | 9 Suppl 12(Suppl 12) |
| ページ | S16 |
| 公開日 | 2008-12-12 |
| DOI | 10.1186/1471-2105-9-S12-S16 |
| PII | 1471-2105-9-S12-S16 |
| PMID | 19091015 |
| PMC | PMC2638156 |
| MeSH | Alcohol Dehydrogenase / chemistry Alcohol Dehydrogenase / physiology* Animals CHO Cells Computational Biology / methods* Cricetinae Cricetulus Mice Microscopy, Confocal Models, Biological PPAR alpha / metabolism Peptides / chemistry Peroxisomal Targeting Signal 2 Receptor Peroxisomes / metabolism* Protein Structure, Tertiary Receptors, Cytoplasmic and Nuclear / chemistry |
| IF | 3.242 |
| 引用数 | 9 |
| WOS 分野 | MATHEMATICAL & COMPUTATIONAL BIOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMICAL RESEARCH METHODS |
| リソース情報 | |
| ヒト・動物細胞 | CHO-K1(RCB0285) |