論文 - 詳細
| RRC ID | 67786 |
|---|---|
| 著者 | Iizumi T, Mizumoto M, Nakamura K. |
| タイトル | A bioluminescence assay using Nitrosomonas europaea for rapid and sensitive detection of nitrification inhibitors. |
| ジャーナル | Appl Environ Microbiol |
| Abstract |
An expression vector for the luxAB genes, derived from Vibrio harveyi, was introduced into Nitrosomonas europaea. Although the recombinant strain produced bioluminescence due to the expression of the luxAB genes under normal growing conditions, the intensity of the light emission decreased immediately, in a time-and dose-dependent manner, with the addition of ammonia monooxygenase inhibitors, such as allylthiourea, phenol, and nitrapyrin. When whole cells were challenged with several nitrification inhibitors and toxic compounds, a close relationship was found between the change in the intensity of the light emission and the level of ammonia-oxidizing activity. The response of bioluminescence to the addition of allylthiourea was considerably faster than the change in the ammonia-oxidizing rate, measured as both the O2 uptake and NO2- production rates. The bioluminescence of cells inactivated by ammonia monooxygenase inhibitor was recovered rapidly by the addition of certain substrates for hydroxylamine oxidoreductase. These results suggested that the inhibition of bioluminescence was caused by the immediate decrease of reducing power in the cell due to the inactivation of ammonia monooxygenase, as well as by the destruction of other cellular metabolic pathways. We conclude that the assay system using luminous Nitrosomonas can be applied as a rapid and sensitive detection test for nitrification inhibitors, and it will be used to monitor the nitrification process in wastewater treatment plants. |
| 巻・号 | 64(10) |
| ページ | 3656-62 |
| 公開日 | 1998-10-1 |
| DOI | 10.1128/AEM.64.10.3656-3662.1998 |
| PMID | 9758781 |
| PMC | PMC106494 |
| MeSH | Enzyme Inhibitors / pharmacology* Genes, Bacterial Kinetics Luciferases / genetics Luciferases / metabolism* Luminescence Nitrites / metabolism* Nitrosomonas / drug effects* Nitrosomonas / physiology* Oxidoreductases / antagonists & inhibitors* Oxygen Consumption / drug effects Phenol / pharmacology Picolines / pharmacology Plasmids Restriction Mapping Thiourea / analogs & derivatives Thiourea / pharmacology Transformation, Bacterial Vibrio / enzymology Vibrio / genetics* |
| IF | 4.016 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pHLUX20 (RDB18951) |