論文 - 詳細
| RRC ID | 67847 |
|---|---|
| 著者 | Fuchi Y, Murase H, Kai R, Kurata K, Karasawa S, Sasaki S. |
| タイトル | Artificial Host Molecules to Covalently Capture 8-Nitro-cGMP in Neutral Aqueous Solutions and in Cells. |
| ジャーナル | Bioconjug Chem |
| Abstract |
New 1,3-diazaphenoxazine derivatives (nitroG-Grasp-Guanidine, NGG) have been developed to covalently capture 8-nitro-cGMP in neutral aqueous solutions, which furnish a thiol reactive group to displace the 8-nitro group and a guanidine unit for interaction with the cyclic phosphate. The thiol group was introduced to the 1,3-diazaphenoxazine skeleton through a 2-aminobenzylthiol group (NGG-H) and its 4-methyl (NGG-pMe) and 6-methyl (NGG-oMe) substituted derivatives. The covalent adducts were formed between the NGG derivatives and 8-nitro-cGMP in neutral aqueous solutions. Among the NGG derivatives, the one with the 6-methyl group (NGG-oMe) exhibited the most efficient capture reaction. Furthermore, NGG-H showed a cell permeability into HEK-293 and RAW 264.7 cells and reduced the intracellular 8-nitro-cGMP level. The NGG derivatives developed in this study would become a valuable tool to study the intracellular role of 8-nitro-cGMP. |
| 巻・号 | 32(2) |
| ページ | 385-393 |
| 公開日 | 2021-2-17 |
| DOI | 10.1021/acs.bioconjchem.1c00012 |
| PMID | 33529519 |
| MeSH | Animals Cyclic GMP / analogs & derivatives* Cyclic GMP / chemistry Cyclic GMP / metabolism HEK293 Cells Humans Mice RAW 264.7 Cells Spectrum Analysis / methods Water |
| IF | 4.031 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 5 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |