| RRC ID |
6785
|
| 著者 |
Wakao H, Wakao R, Sakata S, Iwabuchi K, Oda A, Fujita H.
|
| タイトル |
In vitro induction of natural killer T cells from embryonic stem cells prepared using somatic cell nuclear transfer.
|
| ジャーナル |
FASEB J
|
| Abstract |
The ectopic expression of the Notch receptor ligand delta-like 1 on stromal cells allows the induction of T cells from embryonic stem cells (ESCs). However, these in vitro-generated T cells are not transplantable because they are too immature to mount an immune response in an immunocompromised animal. We efficiently generated a subset of T cells called invariant natural killer T (iNKT) cells from ESCs derived from peripheral iNKT cells using somatic cell nuclear transfer (ntESCs). These iNKT cells matured autonomously in vivo and exhibited an adjuvant effect accompanying the production of interferon-gamma in an antigen-specific manner. This adjuvant effect culminated in the inhibition of inoculated tumor cell growth. Our results indicate that ntESC-derived iNKT cells are transplantable lymphocytes that will be beneficial for the induction of immune tolerance and the treatment of autoimmune diseases, tumors, and infections.
|
| 巻・号 |
22(7)
|
| ページ |
2223-31
|
| 公開日 |
2008-7-1
|
| DOI |
10.1096/fj.07-104687
|
| PII |
fj.07-104687
|
| PMID |
18323403
|
| MeSH |
Animals
CD3 Complex / genetics
Cell Nucleus / immunology*
DNA-Binding Proteins / genetics
Embryonic Stem Cells / cytology
Embryonic Stem Cells / immunology*
GATA3 Transcription Factor / genetics
Humans
Killer Cells, Natural / immunology*
Nuclear Proteins / genetics
Nuclear Transfer Techniques
Receptors, Interleukin-7 / genetics
Reverse Transcriptase Polymerase Chain Reaction
|
| IF |
4.966
|
| 引用数 |
6
|
|
WOS 分野
|
BIOLOGY
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
OP9(RCB1124) |
