RRC ID 67852
著者 Thongsiri C, Nagai-Yoshioka Y, Yamasaki R, Adachi Y, Usui M, Nakashima K, Nishihara T, Ariyoshi W.
タイトル Schizophyllum commune β-glucan: Effect on interleukin-10 expression induced by lipopolysaccharide from periodontopathic bacteria.
ジャーナル Carbohydr Polym
Abstract β-glucans are potent immunomodulators, with effects on innate and adaptive immune responses via dectin-1 as the main receptor. In this study, we investigated the biological effect of β-glucan from Schizophyllum commune, called Schizophyllan (SPG) on Interleukin-10 (IL-10) expression induced by a lipopolysaccharide (LPS) from Aggregatibacter actinomycetemcomitans in murine macrophages (J774.1). SPG and dectin-1 interaction up-regulates LPS-induced IL-10 expression. The regulative effect of SPG on IL-10 expression is dependent on prolongation of nuclear translocation activity of nuclear factor-kappa B (NF-κBα) pathway induced by LPS. We also found that LPS-induced phosphorylation of mitogen- and stress-activated protein kinase 1 (MSK1) and cAMP-responsive-element-binding protein (CREB), followed by up-regulation of IL-10, was stimulated by SPG priming via activation of the spleen tyrosine kinase (Syk). Our data indicate that SPG augments the anti-inflammatory response in murine macrophages which can be useful to create an intervention for periodontal disease treatment.
巻・号 253
ページ 117285
公開日 2021-2-1
DOI 10.1016/j.carbpol.2020.117285
PII S0144-8617(20)31458-2
PMID 33278951
MeSH Adjuvants, Immunologic / metabolism Adjuvants, Immunologic / pharmacology* Aggregatibacter actinomycetemcomitans / chemistry* Animals Fungal Polysaccharides / metabolism Fungal Polysaccharides / pharmacology* Interleukin-10 / metabolism* Lectins, C-Type / metabolism Lipopolysaccharides / pharmacology* Macrophages / drug effects Macrophages / immunology* Mice NF-kappa B / metabolism Pasteurellaceae Infections / drug therapy Pasteurellaceae Infections / microbiology Periodontal Diseases / drug therapy Periodontal Diseases / microbiology Phosphorylation / drug effects Schizophyllum / chemistry* Signal Transduction / drug effects Sizofiran / metabolism Sizofiran / pharmacology*
IF 7.182
リソース情報
ヒト・動物細胞 J774.1(RCB0434)