Reference - Detail
| RRC ID | 67857 |
|---|---|
| Author | Sun Y, Ito S, Nishio N, Tanaka Y, Chen N, Isobe K. |
| Title | Acrolein induced both pulmonary inflammation and the death of lung epithelial cells. |
| Journal | Toxicol Lett |
| Abstract |
Acrolein, a compound found in cigarette smoke, is a major risk factor for respiratory diseases. Previous research determined that both acrolein and cigarette smoke produced reactive oxygen species (ROS). As many types of pulmonary injuries are associated with inflammation, this study sought to ascertain the extent to which exposure to acrolein advanced inflammatory state in the lungs. Our results showed that intranasal exposure of mice to acrolein increased CD11c(+)F4/80(high) macrophages in the lungs and increased ROS formation via induction of NF-κB signaling. Treatment with acrolein activated macrophages and led to their increased production of ROS and expression of several key pro-inflammatory cytokines. In in vitro studies, acrolein treatment of bone marrow-derived GM-CSF-dependent immature macrophages (GM-IMs), activated the cells and led to their increased production of ROS and expression of several key pro-inflammatory cytokines. Acrolein treatment of macrophages induced apoptosis of lung epithelial cells. Inclusion of an inhibitor of ROS formation markedly decreased acrolein-mediated macrophage activation and reduced the extent of epithelial cell death. These results indicate that acrolein can cause lung damage, in great part by mediating the increased release of pro-inflammatory cytokines/factors by macrophages. |
| Volume | 229(2) |
| Pages | 384-92 |
| Published | 2014-9-2 |
| DOI | 10.1016/j.toxlet.2014.06.021 |
| PII | S0378-4274(14)00266-5 |
| PMID | 24999835 |
| MeSH | Acrolein / toxicity* Animals Apoptosis / drug effects* Blotting, Western Cytokines / analysis Cytokines / biosynthesis Female Flow Cytometry Lung / chemistry Lung / cytology Lung / drug effects* Macrophage Activation / drug effects Mice Mice, Inbred C57BL NF-kappa B / drug effects Pneumonia / chemically induced* Reactive Oxygen Species / analysis Real-Time Polymerase Chain Reaction Respiratory Mucosa / drug effects* Signal Transduction / drug effects Transcription Factor RelA / drug effects |
| IF | 3.569 |
| Altmetric score |
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| The most frequently cited source | |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
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| Human and Animal Cells | LLC(RCB0558) |