Reference - Detail
|Author||Oka H, Mori M, Kihara H.|
|Title||F-spondin inhibits migration and differentiation of osteoclastic precursors.|
BACKGROUND:Clinically, severe cemental resorption is a rare consequence of periodontitis, although alveolar bone resorption by osteoclasts is one of the main pathologic changes. F-spondin is a secreted neuronal glycoprotein that localizes to the cementum. F-spondin is among the cementum-specific factors in periodontal tissue that have been reported. However, the effects of F-spondin on osteoclastogenesis have not yet been established. We examined the effects of F-spondin on stages of osteoclastogenesis, migration, and differentiation in a mouse osteoclastic precursor model, RAW 264 cells.
METHODS:RAW 264 cells were treated with recombinant F-spondin. Macrophage colony stimulating factor (M-CSF)-induced cell migration was examined by migration assay performed with cell culture inserts. Osteoclastic differentiation was measured by counting tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells.
RESULTS:In a transmigration assay, F-spondin significantly downregulated M-CSF-induced cell migration. Further, F-spondin significantly reduced the number of receptor activator of nuclear factor-kappa B ligand-induced TRAP-positive multinucleated cells. The receptor-associated protein, an antagonist of the low-density lipoprotein (LDL) receptor family, blocked the effects of F-spondin on M-CSF-induced migration. The suppressive effect of F-spondin on M-CSF-induced cell migration was blocked by knockdown of LDL receptor-related protein 8 (LRP8), a member of the LDL receptor family.
CONCLUSIONS:Our findings suggest that F-spondin downregulates recruitment to the root side of periodontal tissue via LRP8 and inhibits differentiation of osteoclastic precursors. It is suggested that F-spondin is essential to protect the root surface from resorption.
|MeSH||Alveolar Bone Loss / metabolism* Animals Cell Differentiation / physiology Cell Line Cell Movement / physiology Dental Cementum / metabolism* Dental Cementum / physiopathology Down-Regulation Extracellular Matrix Proteins / pharmacology Extracellular Matrix Proteins / physiology* Gene Knockdown Techniques LDL-Receptor Related Proteins / genetics LDL-Receptor Related Proteins / metabolism Macrophage Colony-Stimulating Factor / genetics Macrophages / cytology Mice Osteoclasts / cytology* Osteoclasts / drug effects Osteoclasts / metabolism RANK Ligand / metabolism Receptors, LDL / antagonists & inhibitors Recombinant Proteins|
|Human and Animal Cells||RAW 264(RCB0535)|