Reference - Detail
|Author||Uehara K, Takahashi A, Watanabe M, Nomura Y.|
|Title||Shark protein improves bone mineral density in ovariectomized rats and inhibits osteoclast differentiation.|
OBJECTIVES:Fish proteins are potential sources of natural medicines and food additives. There are many studies being performed to develop underutilized fish proteins. Therefore, the aim of this study was to determine how shark protein functions as a dietary supplement for bone health.
METHODS:Three groups of ovariectomized (OVX) rats were fed different diets containing 20% casein protein, 20% shark protein, or 20% cod protein for 4 wk. Bone mineral density of the right femur was measured by dual-energy x-ray absorptiometry and quantitative computed tomography. Furthermore, we prepared low-molecular-weight peptides from shark protein using protease for in vitro studies. Calcitriol was added to bone marrow cells and the receptor activator of the nuclear factor-κB ligand was added to RAW264 cells. After 7 d, the number of tartrate-resistant acid phosphatase-positive cells was counted.
RESULTS:In the shark protein-fed group, bone mineral density of the femur epiphysis was higher than that of the casein protein-fed group. In particular, the shark protein-fed group showed an increase in bone mineral density, represented mainly by trabecular bone. Shark protein hydrolysates inhibited osteoclast formation in bone marrow cells and RAW264 cells.
CONCLUSIONS:These results suggest that shark protein might suppress the bone loss caused by estrogen deficiency through the suppression of osteoclast formation.
|MeSH||Absorptiometry, Photon Acid Phosphatase / drug effects Acid Phosphatase / metabolism Animals Bone Density / drug effects* Bone Marrow Cells / drug effects Bone Marrow Cells / metabolism Calcitriol / pharmacology Caseins / chemistry Caseins / pharmacology Cell Differentiation / drug effects* Cell Line, Tumor Female Femur / drug effects Femur / metabolism Fish Proteins / chemistry Fish Proteins / pharmacology* Isoenzymes / drug effects Isoenzymes / metabolism Mice Molecular Weight NF-kappa B / metabolism Osteoclasts / drug effects* Osteoclasts / metabolism Ovariectomy Protein Hydrolysates / chemistry Protein Hydrolysates / pharmacology* Rats Rats, Wistar Sharks* Tartrate-Resistant Acid Phosphatase|
|Human and Animal Cells||RAW 264(RCB0535)|