RRC ID 679
Author Xu JH, Kurata N, Akimoto M, Ohtsubo H, Ohtsubo E.
Title Identification and characterization of Australian wild rice strains of Oryza meridionalis and Oryza rufipogon by SINE insertion polymorphism.
Journal Genes Genet Syst
Abstract Of the rice species with an AA genome, Oryza meridionalis has been identified in northern Australia as a species of the annual type, among those previously classified as Oryza perennis, Oryza rufipogon or Oryza nivara. This notion has, however, led to some confusion to determine which strains belong to O. meridionalis and how different these strains are from the O. rufipogon strains of the annual type. In this paper, we examined Australian wild rice strains for the presence or absence of p-SINE1 members, which have been used for identification of the strains of species with the AA genome, by PCR using primers that hybridize to the sequences flanking each p-SINE1 member. The rice strains examined include perennial and annual strains, which have previously been described as O. rufipogon. We found that all the annual strains and other strains, whose types have not been determined, have p-SINE1 members that are specifically present at the corresponding loci in the standard strains of O. meridionalis, but do not have those which are specifically present at the corresponding loci in the strains of the other species with the AA genome. The perennial strains, however, have p-SINE1 members that are specifically present at the corresponding loci in the standard O. rufipogon strains of either the annual or the perennial type, but do not have those which are specifically present at the corresponding loci in the strains of the other species with the AA genome, including O. meridionalis. These findings support the previous notion that O. meridionalis consists of the annual strains and is a distinct species from O. rufipogon. The p-SINE1 members used in this study appear to be very useful for classification of any wild rice strains of the AA-genome species, even when one has limited knowledge of morphology, taxonomy, physiology, and biochemistry of rice strains.
Volume 80(2)
Pages 129-34
Published 2005-4-1
DOI 10.1266/ggs.80.129
PII JST.JSTAGE/ggs/80.129
PMID 16172525
MeSH Australia Base Sequence DNA, Plant / chemistry Genome, Plant Molecular Sequence Data Oryza / classification* Oryza / genetics* Polymerase Chain Reaction Polymorphism, Genetic* Short Interspersed Nucleotide Elements / genetics*
IF 0.859
Times Cited 18
WOS Category GENETICS & HEREDITY BIOCHEMISTRY & MOLECULAR BIOLOGY
Resource
Rice W0106 W0630 W0120. W1945 W1236 W2078 W2099 W2109 W2119 W2110 W1297 W1627 W1625 W1635 W2069 W2079 W2130 W2077 W2081 W2100 W2112 W2116 W2123 W0025 W1581 W1588 W1191 W1192 W0708 W1452