論文 - 詳細
| RRC ID | 67919 |
|---|---|
| 著者 | Miyashita K, Oyama T, Sakuta T, Tokuda M, Torii M. |
| タイトル | Anandamide induces matrix metalloproteinase-2 production through cannabinoid-1 receptor and transient receptor potential vanilloid-1 in human dental pulp cells in culture. |
| ジャーナル | J Endod |
| Abstract |
INTRODUCTION:Anandamide (N-arachidonoylethanolamine [AEA]) is one of the main endocannabinoids. Endocannabinoids are implicated in various physiological and pathologic functions, inducing not only nociception but also regeneration and inflammation. The role of the endocannabinoid system in peripheral organs was recently described. The aim of this study was to investigate the effect of AEA on matrix metalloproteinase (MMP)-2 induction in human dental pulp cells (HPC). METHODS:We examined AEA-induced MMP-2 production and the expression of AEA receptors (cannabinoid [CB] receptor-1, CB2, and transient receptor potential vanilloid-1 [TRPV1]) in HPC by Western blot. MMP-2 concentrations in supernatants were determined by enzyme-linked immunosorbent assay. We then investigated the role of the AEA receptors and mitogen-activated protein kinase in AEA-induced MMP-2 production in HPC. RESULTS:AEA significantly induced MMP-2 production in HPC. HPC expressed all 3 types of AEA receptor (CB1, CB2, and TRPV1). AEA-induced MMP-2 production was blocked by CB1 or TRPV1 antagonists and by small interfering RNA for CB1 or TRPV1. Furthermore, c-Jun N-terminal kinase inhibitor also reduced MMP-2 production. CONCLUSIONS:We demonstrated for the first time that AEA induced MMP-2 production via CB1 and TRPV1 in HPC. |
| 巻・号 | 38(6) |
| ページ | 786-90 |
| 公開日 | 2012-6-1 |
| DOI | 10.1016/j.joen.2012.02.025 |
| PII | S0099-2399(12)00219-1 |
| PMID | 22595113 |
| MeSH | Arachidonic Acids / pharmacology Arachidonic Acids / physiology* Cells, Cultured Dental Pulp / cytology Dental Pulp / metabolism* Endocannabinoids Humans JNK Mitogen-Activated Protein Kinases / antagonists & inhibitors JNK Mitogen-Activated Protein Kinases / metabolism Matrix Metalloproteinase 2 / biosynthesis* Polyunsaturated Alkamides / pharmacology RNA, Small Interfering / genetics Receptor, Cannabinoid, CB1 / metabolism* TRPV Cation Channels / metabolism* Transfection |
| IF | 3.118 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) HL60(RCB0041) |