| RRC ID |
67941
|
| 著者 |
Miki T, Iguchi M, Akiba K, Hosono M, Sobue T, Danbara H, Okada N.
|
| タイトル |
Chromobacterium pathogenicity island 1 type III secretion system is a major virulence determinant for Chromobacterium violaceum-induced cell death in hepatocytes.
|
| ジャーナル |
Mol Microbiol
|
| Abstract |
Chromobacterium violaceum is a Gram-negative bacterium that causes fatal septicaemia in humans and animals. C. violaceum ATCC 12472 possesses genes associated with two distinct type III secretion systems (T3SSs). One of these systems is encoded by Chromobacterium pathogenicity islands 1 and 1a (Cpi-1/-1a), another is encoded by Chromobacterium pathogenicity island 2 (Cpi-2). Here we show that C. violaceum causes fulminant hepatitis in a mouse infection model, and Cpi-1/-1a-encoded T3SS is required for its virulence. In addition, using C. violaceum strains with defined mutations in the genes that encode the Cpi-1/-1a or Cpi-2 locus in combination with cultured mammalian cell lines, we found that C. violaceum is able to induce cytotoxicity in a Cpi-1/-1a-dependent manner. Characterization of Chromobacterium-induced cytotoxicity revealed that cell lysis by C. violaceum infection involves the formation of pore structures on the host cell membrane, as demonstrated by protection by cytotoxicity in the presence of osmoprotectants. Finally, we demonstrated that CipB, a Cpi-1/-1a effector, is implicated in translocator-mediated pore formation and the ability of CipB to form a pore is essential for Chromobacterium-induced cytotoxicity. These results strongly suggest that Cpi-1/-1a-encoded T3SS is a virulence determinant that causes fatal infection by the induction of cell death in hepatocytes.
|
| 巻・号 |
77(4)
|
| ページ |
855-72
|
| 公開日 |
2010-8-1
|
| DOI |
10.1111/j.1365-2958.2010.07248.x
|
| PII |
MMI7248
|
| PMID |
20545857
|
| MeSH |
Animals
Bacterial Secretion Systems*
Bodily Secretions
Cell Death
Chromobacterium / genetics*
Chromobacterium / growth & development*
Chromobacterium / pathogenicity
Disease Models, Animal
Genomic Islands*
Gram-Negative Bacterial Infections / microbiology
Gram-Negative Bacterial Infections / pathology
Hepatitis, Animal / microbiology
Hepatitis, Animal / pathology
Hepatocytes / microbiology
Hepatocytes / physiology
Mice
Virulence
Virulence Factors / genetics
Virulence Factors / metabolism*
|
| IF |
3.418
|
| リソース情報 |
| ヒト・動物細胞 |
Hepa 1-6(RCB1638)
Hep G2(RCB1648) |
