RRC ID 67944
Author Yang DW, Mok JW, Telerman SB, Amson R, Telerman A, Choi KW.
Title Topoisomerase II is regulated by translationally controlled tumor protein for cell survival during organ growth in Drosophila.
Journal Cell Death Dis
Abstract Regulation of cell survival is critical for organ development. Translationally controlled tumor protein (TCTP) is a conserved protein family implicated in the control of cell survival during normal development and tumorigenesis. Previously, we have identified a human Topoisomerase II (TOP2) as a TCTP partner, but its role in vivo has been unknown. To determine the significance of this interaction, we examined their roles in developing Drosophila organs. Top2 RNAi in the wing disc leads to tissue reduction and caspase activation, indicating the essential role of Top2 for cell survival. Top2 RNAi in the eye disc also causes loss of eye and head tissues. Tctp RNAi enhances the phenotypes of Top2 RNAi. The depletion of Tctp reduces Top2 levels in the wing disc and vice versa. Wing size is reduced by Top2 overexpression, implying that proper regulation of Top2 level is important for normal organ development. The wing phenotype of Tctp RNAi is partially suppressed by Top2 overexpression. This study suggests that mutual regulation of Tctp and Top2 protein levels is critical for cell survival during organ development.
Volume 12(9)
Pages 811
Published 2021-8-27
DOI 10.1038/s41419-021-04091-y
PII 10.1038/s41419-021-04091-y
PMID 34453033
PMC PMC8397738
MeSH Animals Cell Survival / genetics DNA Topoisomerases, Type II / metabolism* Drosophila Proteins / metabolism* Drosophila melanogaster / cytology* Drosophila melanogaster / genetics Drosophila melanogaster / growth & development* Epistasis, Genetic Female Green Fluorescent Proteins / metabolism Intracellular Signaling Peptides and Proteins / metabolism* Male Organogenesis* / genetics Phenotype RNA Interference Wings, Animal / growth & development
IF 6.304
Resource
Drosophila 10223R-2