RRC ID 67965
Author Hida S, Nagi-Miura N, Adachi Y, Ohno N.
Title Beta-glucan derived from zymosan acts as an adjuvant for collagen-induced arthritis.
Journal Microbiol Immunol
Abstract Collagen-induced arthritis (CIA) is an experimental model of rheumatoid arthritis (RA) and has helped researchers to analyze the pathogenesis of inflammatory joint disease. In classical CIA, Freund's complete adjuvant (FCA), which contains heat-killed Mycobacterium tuberculosis, is used as an adjuvant. In our previous study, we reported that particles of beta-glucan, OX-CA, derived from Candida albicans, acted as a proper adjuvant in the CIA model. In this study, to establish pure beta-glucan as an adjuvant for CIA, we tested a commercially available preparation of Zymosan A (ZYM) and modified its products. beta-Glucan fractions of ZYM were prepared by oxidation with various concentrations of NaClO. The oxidized ZYM (OX-ZYM) was mainly composed of beta-glucan. In this study, we examined its effect as an adjuvant for CIA. DBA/1 mice injected with CII and OX-CA developed arthritis 7-10 days after receiving booster injections; the OX-ZYM fractions induced arthritis with the same time course. 0.01% OX-ZYM (oxidized with a 0.01% NaClO solution) caused arthritis faster than 0.1% OX-ZYM or 0.5% OX-ZYM. In conclusion, beta-glucan derived from ZYM by brief oxidation with NaClO is a suitable adjuvant for a CIA model with anti-CII antibody production.
Volume 50(6)
Pages 453-61
Published 2006-1-1
DOI 10.1111/j.1348-0421.2006.tb03814.x
PII JST.JSTAGE/mandi/50.453
PMID 16785717
MeSH Adjuvants, Immunologic / pharmacology* Animals Arthritis, Experimental / etiology* Cells, Cultured Collagen Type II / immunology* Lipopolysaccharides / pharmacology Macrophage Activation / drug effects Male Mice Mice, Inbred DBA Sodium Hypochlorite / pharmacology Zymosan / pharmacology* beta-Glucans / pharmacology*
IF 1.566
Human and Animal Cells RAW 264(RCB0535)