| RRC ID |
68081
|
| 著者 |
Sasaki J, Hashimoto M, Yamaguchi S, Itoh Y, Yoshimoto I, Matsumoto T, Imazato S.
|
| タイトル |
Fabrication of Biomimetic Bone Tissue Using Mesenchymal Stem Cell-Derived Three-Dimensional Constructs Incorporating Endothelial Cells.
|
| ジャーナル |
PLoS One
|
| Abstract |
The development of technologies to promote vascularization of engineered tissue would drive major developments in tissue engineering and regenerative medicine. Recently, we succeeded in fabricating three-dimensional (3D) cell constructs composed of mesenchymal stem cells (MSCs). However, the majority of cells within the constructs underwent necrosis due to a lack of nutrients and oxygen. We hypothesized that incorporation of vascular endothelial cells would improve the cell survival rate and aid in the fabrication of biomimetic bone tissues in vitro. The purpose of this study was to assess the impact of endothelial cells combined with the MSC constructs (MSC/HUVEC constructs) during short- and long-term culture. When human umbilical vein endothelial cells (HUVECs) were incorporated into the cell constructs, cell viability and growth factor production were increased after 7 days. Furthermore, HUVECs were observed to proliferate and self-organize into reticulate porous structures by interacting with the MSCs. After long-term culture, MSC/HUVEC constructs formed abundant mineralized matrices compared with those composed of MSCs alone. Transmission electron microscopy and qualitative analysis revealed that the mineralized matrices comprised porous cancellous bone-like tissues. These results demonstrate that highly biomimetic bone tissue can be fabricated in vitro by 3D MSC constructs incorporated with HUVECs.
|
| 巻・号 |
10(6)
|
| ページ |
e0129266
|
| 公開日 |
2015-1-1
|
| DOI |
10.1371/journal.pone.0129266
|
| PII |
PONE-D-15-02356
|
| PMID |
26047122
|
| PMC |
PMC4457484
|
| MeSH |
Alkaline Phosphatase / metabolism
Biomimetic Materials / chemistry
Bone and Bones / cytology
Bone and Bones / physiology*
Cells, Cultured
Coculture Techniques
Human Umbilical Vein Endothelial Cells / cytology*
Human Umbilical Vein Endothelial Cells / metabolism
Human Umbilical Vein Endothelial Cells / ultrastructure
Humans
Mesenchymal Stem Cells / cytology*
Mesenchymal Stem Cells / metabolism
Mesenchymal Stem Cells / ultrastructure
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Minerals / metabolism
Osteogenesis
Platelet Endothelial Cell Adhesion Molecule-1 / metabolism
Spectroscopy, Fourier Transform Infrared
Time Factors
Tissue Engineering / methods*
Tissue Scaffolds / chemistry
|
| IF |
2.74
|
| リソース情報 |
| ヒト・動物細胞 |
Human mesenchymal stem cells |
