論文 - 詳細
| RRC ID | 68084 |
|---|---|
| 著者 | Yunoki S, Hatayama H, Ebisawa M, Kondo E, Yasuda K. |
| タイトル | A novel fabrication method to create a thick collagen bundle composed of uniaxially aligned fibrils: an essential technology for the development of artificial tendon/ligament matrices. |
| ジャーナル | J Biomed Mater Res A |
| Abstract |
In this study, we developed a fabrication method for thick collagen gel bundles comprising uniaxially aligned fibrils of sufficient size for filling defects in ligament tissues. The fabrication involved rotary shearing to dense collagen sols using a rheometer and then warming them from 23°C to 37°C to trigger gelation upon rotation. Gelation due to collagen fibril formation was accelerated by increased concentrations of neutral phosphate buffer, and fibril alignment occurred within 20 s during the early stage of rapid gelation. Fabrication of gels was completed with slippage between gels and the movable upper plate, and well-aligned fibrils along the rotation direction were observed in the marginal regions of disc-shaped gels. Gel thickness could be increased from 1 to 3 mm with homogeneous alignment of fibrils in the entire sample. The alignment of fibrils improved mechanical properties against tensile loads that were placed parallel to the alignment axis. Elongation of cultured fibroblast along the alignment was observed on the gels. The present method will enable the bottom-up fabrication of an artificial tendon for ligament reconstruction and repair. |
| 巻・号 | 103(9) |
| ページ | 3054-65 |
| 公開日 | 2015-9-1 |
| DOI | 10.1002/jbm.a.35440 |
| PMID | 25727567 |
| MeSH | Animals Biocompatible Materials / chemistry Cells, Cultured Collagen / chemistry* Fibroblasts / cytology Gels Humans Ligaments / injuries* Ligaments / surgery Materials Testing Tendon Injuries / surgery* Tensile Strength Tissue Engineering / methods* Tissue Scaffolds / chemistry* |
| IF | 3.525 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 9 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | NIH/3T3(RCB2767) |